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RNAi Experiment: PF:GL1_3F5 Ace View of PF:GL1_3F5
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C32F10.5 (hmg-3)
Description of "C32F10.5"  Associated GO Term : DNA binding
KOG Annotation  Nucleosome-binding factor SPN, POB3 subunit
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (I:5816156..5817320)
Transcript overlap: 1077 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 1035
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.92
Wormbase (BLAST) RNAi primary
External "C32F10.5" Links  Wormbase NCBI
Gene Inhibited  T20B12.8 (hmg-4)
Description of "T20B12.8"  hmg-4 encodes a protein with strong similarity to the highly conserved high mobility group protein SSRP1 (structure-specific DNA recognition protein); by homology, HMG-4 is predicted to be a member of the FACT (facilitates chromatin transcription) complex that functions as a transcription elongation factor; RNAi experiments indicate that hmg-4 is required for locomotion and larval development, and required redundantly with hmg-3, its paralog, for embryonic development.
KOG Annotation  Nucleosome-binding factor SPN, POB3 subunit
What's a KOG?
Evidence for Inhibition 
Sliding Window Raw score: 90
Relative score: 0.087 (Best raw score= for Gene )
Specificity index: 0.08
Wormbase (BLAST) RNAi secondary
External "T20B12.8" Links  Wormbase NCBI
PF:GL1_3F5 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 %
Larval  Gro Observed in >=10% of progeny.
Lva Observed in >=10% of progeny.
Adult  Stp Observed in >=10% of progeny.
Phenotype  Large cytoplasmic granules
Multiple nuclei in 1, 2, or 4-cell embryos
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_3F5 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_C32F10.5 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of T20B12.8 Browse using GBrowse
Gene Graph
Gene Plot of C32F10.5 Browse using GBrowse
Gene Graph