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RNAi Experiment: PF:GL1_3G3 Ace View of PF:GL1_3G3
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C25A1.9
Description of "C25A1.9"  Associated GO Term : calcium ion binding
KOG Annotation  Protein phosphatase 2 regulatory subunit
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (I:10186400..10187509)
Transcript overlap: 840 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 714
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "C25A1.9" Links  Wormbase NCBI
Gene Inhibited  C25A1.10 (dao-5)
Description of "C25A1.10"  dao-5 encodes a predicted nucleolar phosphoprotein related to Saccharomyces cerevisiae SRP40 and the vertebrate Nopp140 proteins that may play a role in rRNA gene transcription and nucleolar structural organization; dao-5 expression is negatively regulated by the DAF-2/insulin-like receptor and positively regulated by the DAF-16/fork-head transcription factor, suggesting that dao-5 may be involved in life-span determination; dao-5 is strongly expressed in dauer larvae.
Evidence for Inhibition 
ePCR Transcript overlap: 8 nt Explain types of evidence for RNAi inhibition
External "C25A1.10" Links  Wormbase NCBI
PF:GL1_3G3 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 %
Larval  Lva Observed in <10% of progeny.
Adult  Stp Observed in <10% of progeny.
Pleiotropic
Phenotype  Aberrant AB or P1 spindle orientation
Aberrant cell-cell contacts at the four-cell stage
Cytokinesis defect
Ectopic cleavage furrows
Excessive blebbing
Loose mitotic furrow
Multiple nuclei in 1, 2, or 4-cell embryos
P0 spindle positioning defect
P1 aster abnormal
P2 and EMS divide synchronously
Pronuclear breakdown asynchronous
Pronuclei meet more centrally
Pseudocleavage absent
Synchronous second division
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_3G3 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_C25A1.9 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of C25A1.10 Browse using GBrowse
Gene Graph
Gene Plot of C25A1.9 Browse using GBrowse
Gene Graph