Ace View of PF:GL1_4D12 | ||||||
Gene Inhibited | K08E7.3 (let-99) | |||||
Description of "K08E7.3" | let-99 encodes a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); LET-99 has no obvious homologs in non-nematodes, but has a truncated paralog (LRG-1) in C. elegans; LET-99 is required for the mitotic spindle to be properly oriented with respect to the axis of cellular polarity, both during anterior migration and rotation of the nuclear-centrosome complex and during anaphase; let-99 mutants exhibit hyperactive nuclear movement and abnormal anaphase spindle pole behavior; LET-99 is mislocalized in par-2 and par-3 mutants; like other proteins containing DEP domains, LET-99 may act as part of a G protein signalling pathway (e.g., the one controlling spindle position). | |||||
Evidence for Inhibition |
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External "K08E7.3" Links | ||||||
Gene Inhibited | F55H2.4 (lrg-1) | |||||
Description of "F55H2.4" | lrg-1 encodes a truncated paralog of let-99, a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); lrg-1(RNAi) animals have no obvious phenotype; since the nucleotide similarity of lrg-1 to let-99 extends beyond the lrg-1 CDS, and since lrg-1 has no obvious function in RNAi assays, this gene may be decaying towards pseudogene status. | |||||
Evidence for Inhibition |
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External "F55H2.4" Links | ||||||
Embryonic | Emb Penetrance 80 - 100 % | |||||
Larval | Lva
Observed in >=10% of progeny. |
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Adult | Stp
Observed in >=10% of progeny. |
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Phenotype | Aberrant cell-cell contacts at the four-cell stage Delayed P0 spindle rotation Division axis of ABa or ABp aberrant Large cytoplasmic granules P0 spindle does not rotate P0 spindle positioning defect Pronuclei meet more centrally Synchronous second division |
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Movies/Stacks/Images | ||||||
Laboratory | KK | |||||
RNAi Reagent & Type | sjj_F55H2.4 (PCR_reagent) | |||||
Remark | PCR product contains flanking T7 promoter sequences | |||||
Reference | Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" | |||||
Delivery Method | Injection | |||||
Date | 2002-11-19 | |||||
Gene Plot of K08E7.3 | ||||||
Gene Plot of F55H2.4 | ||||||