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RNAi Experiment: PF:GL1_4E4 Ace View of PF:GL1_4E4
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C08B6.9 (aos-1)
Description of "C08B6.9"  aos-1 encodes the C. elegans ortholog of Saccharomyces cerevisiae Aos1p and human SUMO-1 activating enzyme E1 N subunit; by homology, AOS-1 is predicted to form, with a catalytic subunit, UBA-2, a heterodimeric enzyme that activates the ubiquitin-like protein SMO-1/SUMO in preparation for its covalent attachment to target proteins to regulate their subcellular localization and/or stability; in C. elegans, AOS-1 is required for embryonic and larval development, as well as for proper Hox gene regulation; uba-2/aos-1(RNAi) animals that survive embryogenesis show ectopic expression of EGL-5 and ectopic anterior sensory ray formation.
KOG Annotation  SMT3/SUMO-activating complex, AOS1/RAD31 component
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (V:10131850..10133039)
Transcript overlap: 887 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 782
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "C08B6.9" Links  Wormbase NCBI
Gene Inhibited  C08B6.7
KOG Annotation  WD40 protein DMR-N9
What's a KOG?
Evidence for Inhibition 
ePCR Transcript overlap: 53 nt Explain types of evidence for RNAi inhibition
External "C08B6.7" Links  Wormbase NCBI
PF:GL1_4E4 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 % Sle
Larval  Lva Observed in >=10% of progeny.
Phenotype  Abnormal size of pronucleus
Multiple cavities
P0 spindle does not rotate
P0 spindle positioning defect
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_4E4 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_C08B6.9 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of C08B6.9 Browse using GBrowse
Gene Graph
Gene Plot of C08B6.7 Browse using GBrowse
Gene Graph