Ace View of PF:GL1_4G9 | |||
Gene Inhibited | F10B5.6 (emb-27) | ||
Description of "F10B5.6" | emb-27 encodes a tetratricopeptide repeat (TPR)-containing protein that is the C. elegans ortholog of the anaphase-promoting complex (APC) subunit APC-6 (CDC16); required maternally and paternally, EMB-27 activity is essential for proper execution of the metaphase-to-anaphase transition during meiosis (both oogenesis and spermatogenesis) and mitosis (during germline proliferation); EMB-27 activity is also critical for embryonic anterior-posterior (A-P) axis formation, production of the secreted eggshell, and normal vulval development; in establishing the embryonic A-P axis, EMB-27 likely acts upstream of the separin protease to ensure proper localization of PAR-3 and PAR-2 to the anterior and posterior cortices, respectively. | ||
KOG Annotation  | Anaphase-promoting complex (APC), Cdc16 subunit |
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Evidence for Inhibition | |||
External "F10B5.6" Links | |||
Embryonic | Emb Penetrance 80 - 100 % | ||
Adult | Stp
Observed in >=10% of progeny. No developing embryos seen 48 hours after dsRNA injection Reduced fecundity of injected worm Ste |
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Phenotype | Catastrophic one-cell arrest Osmotically/ pressure sensitive |
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Movies/Stacks/Images | |||
Laboratory | KK | ||
RNAi Reagent & Type | sjj_F10B5.6 (PCR_reagent) | ||
Remark | PCR product contains flanking T7 promoter sequences | ||
Reference | Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" | ||
Delivery Method | Injection | ||
Date | 2002-11-19 | ||
Gene Plot of F10B5.6 | |||