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RNAi Experiment: PF:GL1_4G9 Ace View of PF:GL1_4G9
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F10B5.6 (emb-27)
Description of "F10B5.6"  emb-27 encodes a tetratricopeptide repeat (TPR)-containing protein that is the C. elegans ortholog of the anaphase-promoting complex (APC) subunit APC-6 (CDC16); required maternally and paternally, EMB-27 activity is essential for proper execution of the metaphase-to-anaphase transition during meiosis (both oogenesis and spermatogenesis) and mitosis (during germline proliferation); EMB-27 activity is also critical for embryonic anterior-posterior (A-P) axis formation, production of the secreted eggshell, and normal vulval development; in establishing the embryonic A-P axis, EMB-27 likely acts upstream of the separin protease to ensure proper localization of PAR-3 and PAR-2 to the anterior and posterior cortices, respectively.
KOG Annotation  Anaphase-promoting complex (APC), Cdc16 subunit
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (II:8160321..8161409)
Transcript overlap: 950 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 866
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "F10B5.6" Links  Wormbase NCBI
PF:GL1_4G9 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 %
Adult  Stp Observed in >=10% of progeny.
No developing embryos seen 48 hours after dsRNA injection
Reduced fecundity of injected worm
Phenotype  Catastrophic one-cell arrest
Osmotically/ pressure sensitive
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_4G9 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_F10B5.6 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of F10B5.6 Browse using GBrowse
Gene Graph