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RNAi Experiment: PF:GL1_5A12 Ace View of PF:GL1_5A12
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C01G8.2 (cln-3.2)
Description of "C01G8.2"  cln-3.2 encodes a predicted transmembrane protein that comprises one of three C. elegans homologs of human CLN3 (OMIM:607042, mutations are associated with Batten disease (also known as juvenile neuronal ceroid lipofuscinosis), a recessively inherited childhood neurodegenerative disorder characterized by progressive loss of vision, seizures, and psychomotor abnormalities); by homology, CLN-3.2 is predicted to localize to Golgi and lysosomal membranes, where its proposed functions include that of a chaperone and/or a regulator of intracellular trafficking; in C. elegans, loss of cln-3.2 activity via mutation or RNA-mediated interference (RNAi) does not result in any obvious abnormalities (nor does loss of all three CLN3 homologs, cln-3.1, cln-3.2, and cln-3.3), suggesting that these genes do not play an essential role in development and/or behavior.
KOG Annotation  Predicted small molecule transporter involved in cellular pH homeostasis (Batten disease protein in human)
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (I:5277224..5278710)
Transcript overlap: 773 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 647
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "C01G8.2" Links  Wormbase NCBI
PF:GL1_5A12 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 15 %
PF:GL1_5A12 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_C01G8.2 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of C01G8.2 Browse using GBrowse
Gene Graph