Ace View of PF:GL1_5C1 | |||
Gene Inhibited | C14B9.4 (plk-1) | ||
Description of "C14B9.4" | plk-1 encodes a serine/threonine polo-like kinase homologous to Drosophila polo and Saccharomyces cerevisiae CDC5; PLK-1 is required for meiotic nuclear envelope breakdown, polar body formation and extrusion, and proper chromosome segregation during meiosis, and additionally, may play a role in germline development; PLK-1 is proposed to phosphorylate REC-8, a meiotic-specific cohesin, thus promoting REC-8 degradation and subsequent separation of sister chromatids; PLK-1 is localized diffusely in the cytoplasm of mature oocytes, but during meiosis I becomes associated with pre-metaphase chromatin and then at anaphase with chromatin and the area between dividing chromatin; in embryos, PLK-1 localizes to centrosomes, metaphase chromatin, and during telophase, to the area between dividing chromatin. | ||
Evidence for Inhibition | |||
External "C14B9.4" Links | |||
Embryonic | Emb Penetrance 100 % | ||
Adult | Ste Reduced fecundity of injected worm |
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Phenotype | Catastrophic one-cell arrest Osmotically/ pressure sensitive Slow cell cycle |
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Movies/Stacks/Images | |||
Laboratory | KK | ||
RNAi Reagent & Type | sjj_C14B9.4 (PCR_reagent) | ||
Remark | PCR product contains flanking T7 promoter sequences | ||
Reference | Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" | ||
Delivery Method | Injection | ||
Date | 2002-11-19 | ||
Gene Plot of C14B9.4 | |||