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RNAi Experiment: PF:GL1_5C1 Ace View of PF:GL1_5C1
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C14B9.4 (plk-1)
Description of "C14B9.4"  plk-1 encodes a serine/threonine polo-like kinase homologous to Drosophila polo and Saccharomyces cerevisiae CDC5; PLK-1 is required for meiotic nuclear envelope breakdown, polar body formation and extrusion, and proper chromosome segregation during meiosis, and additionally, may play a role in germline development; PLK-1 is proposed to phosphorylate REC-8, a meiotic-specific cohesin, thus promoting REC-8 degradation and subsequent separation of sister chromatids; PLK-1 is localized diffusely in the cytoplasm of mature oocytes, but during meiosis I becomes associated with pre-metaphase chromatin and then at anaphase with chromatin and the area between dividing chromatin; in embryos, PLK-1 localizes to centrosomes, metaphase chromatin, and during telophase, to the area between dividing chromatin.
Evidence for Inhibition 
ePCR Status: Unique_ePCR (III:8102629..8103781)
Transcript overlap: 1010 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 926
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "C14B9.4" Links  Wormbase NCBI
PF:GL1_5C1 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 100 %
Adult  Ste
Reduced fecundity of injected worm
Phenotype  Catastrophic one-cell arrest
Osmotically/ pressure sensitive
Slow cell cycle
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_5C1 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_C14B9.4 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of C14B9.4 Browse using GBrowse
Gene Graph