| Ace View of PF:GL1_5G7 | |||||||||
| Gene Inhibited | F18A1.5 | ||||||||
| Description of "F18A1.5" | F18A1.5 encodes the C. elegans ortholog of the replication protein A (RPA) large subunit; by homology, the F18A1.5 gene product is predicted to function as a DNA binding and oligonucleotide oligosaccharide binding (OB) protein that is required for DNA replication, repair, and recombination; large-scale RNAi screens indicate that the product of F18A1.5 is essential for embryonic development, and that in yeast two-hybrid assays interacts with the product of M04F3.1, the RPA medium subunit; in situ hybridization studies indicate that F18A1.5 transcripts are expressed in germ cells. | ||||||||
| KOG Annotation | Single-stranded DNA-binding replication protein A (RPA), large (70 kD) subunit and related ssDNA-binding proteins |
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| Evidence for Inhibition |
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| External "F18A1.5" Links |   |
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| Embryonic | Emb Penetrance 100 % | ||||||||
| Phenotype | Aberrant cell-cell contacts at the four-cell stage Aberrant nuclear reassembly Abnormal size of pronucleus Ectopic cleavage furrows Exaggerated asynchrony Excessive blebbing Multiple maternal pronuclei Slow cell cycle |
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| Movies/Stacks/Images |  |
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| Laboratory | KK | ||||||||
| RNAi Reagent & Type | sjj_F18A1.5 (PCR_reagent) | ||||||||
| Remark | PCR product contains flanking T7 promoter sequences | ||||||||
| Reference | Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" | ||||||||
| Delivery Method | Injection | ||||||||
| Date | 2002-11-19 | ||||||||
| Gene Plot of F18A1.5 | |||||||||
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