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RNAi Experiment: PF:GL1_6D7 Ace View of PF:GL1_6D7
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F33H1.2 (gpd-4)
Description of "F33H1.2"  gpd-4 encodes one of four C. elegans glyceraldehyde-3-phosphate dehydrogenases (GAPDHs); by homology, GPD-4 is predicted to reversibly catalyze the oxidation and phosphorylation of glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate during glycolysis; GPD-4 is required for embryonic and larval development; in C. elegans, GPD-4 and GPD-1 constitute the minor GAPDH isoenzymes and are expressed primarily in embryos, in all cell types.
KOG Annotation  Glyceraldehyde 3-phosphate dehydrogenase
What's a KOG?
Evidence for Inhibition 
ePCR Status: Best_ePCR (II:10171212..10172287)
Transcript overlap: 976 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 696
Relative score: 0.762 (Best raw score= for Gene )
Specificity index: 0.433
Wormbase (BLAST) RNAi primary
External "F33H1.2" Links  Wormbase NCBI
Gene Inhibited  T09F3.3 (gpd-1)
Description of "T09F3.3"  gpd-1 encodes one of four C. elegans glyceraldehyde-3-phosphate dehydrogenases (GAPDHs); by homology, GPD-1 is predicted to reversibly catalyze the oxidation and phosphorylation of glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate during glycolysis; GPD-1 is required for embryogenesis, normal body morphology, and gonad development; in C. elegans, GPD-1 and GPD-4 constitute the minor GAPDH isoenzymes and are expressed primarily in embryos, in all cell types.
KOG Annotation  Glyceraldehyde 3-phosphate dehydrogenase
What's a KOG?
Evidence for Inhibition 
ePCR Status: Best_ePCR (II:10394068..10395143)
Transcript overlap: 976 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 913
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.567
Wormbase (BLAST) RNAi primary
External "T09F3.3" Links  Wormbase NCBI
PF:GL1_6D7 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 %
Adult  Gon Observed in <10% of progeny.
Sma Observed in <10% of progeny.
PF:GL1_6D7 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_T09F3.3 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of T09F3.3 Browse using GBrowse
Gene Graph
Gene Plot of F33H1.2 Browse using GBrowse
Gene Graph