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RNAi Experiment: PF:GL1_6E4 Ace View of PF:GL1_6E4
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  K08E7.3 (let-99)
Description of "K08E7.3"  let-99 encodes a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); LET-99 has no obvious homologs in non-nematodes, but has a truncated paralog (LRG-1) in C. elegans; LET-99 is required for the mitotic spindle to be properly oriented with respect to the axis of cellular polarity, both during anterior migration and rotation of the nuclear-centrosome complex and during anaphase; let-99 mutants exhibit hyperactive nuclear movement and abnormal anaphase spindle pole behavior; LET-99 is mislocalized in par-2 and par-3 mutants; like other proteins containing DEP domains, LET-99 may act as part of a G protein signalling pathway (e.g., the one controlling spindle position).
Evidence for Inhibition 
ePCR Status: Unique_ePCR (IV:12567828..12568978)
Transcript overlap: 1054 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 991
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.854
Wormbase (BLAST) RNAi primary
External "K08E7.3" Links  Wormbase NCBI
Gene Inhibited  F55H2.4 (lrg-1)
Description of "F55H2.4"  lrg-1 encodes a truncated paralog of let-99, a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); lrg-1(RNAi) animals have no obvious phenotype; since the nucleotide similarity of lrg-1 to let-99 extends beyond the lrg-1 CDS, and since lrg-1 has no obvious function in RNAi assays, this gene may be decaying towards pseudogene status.
Evidence for Inhibition 
Sliding Window Raw score: 169
Relative score: 0.171 (Best raw score= for Gene )
Specificity index: 0.146
Wormbase (BLAST) RNAi secondary
External "F55H2.4" Links  Wormbase NCBI
PF:GL1_6E4 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 %
Adult  Stp Observed in >=10% of progeny.
Phenotype  Aberrant AB or P1 spindle orientation
Aberrant cell-cell contacts at the four-cell stage
Delayed P0 spindle rotation
Division axis of ABa or ABp aberrant
Nuclei reform next to cell division remnant
P0 spindle does not rotate
P0 spindle positioning defect
P1 aster abnormal
P2 and EMS divide synchronously
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_6E4 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_K08E7.3 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of K08E7.3 Browse using GBrowse
Gene Graph
Gene Plot of F55H2.4 Browse using GBrowse
Gene Graph