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RNAi Experiment: PF:GL1_7D6 Ace View of PF:GL1_7D6
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F54B3.3
Description of "F54B3.3"  Associated GO Term : nucleotide binding
KOG Annotation  AAA+-type ATPase
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (II:10249728..10250691)
Transcript overlap: 917 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 875
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "F54B3.3" Links  Wormbase NCBI
PF:GL1_7D6 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 %
Larval  Gro Observed in >=10% of progeny.
Lva Observed in >=10% of progeny.
Adult  Ste
Reduced fecundity of injected worm
Phenotype  Aberrant AB or P1 spindle orientation
Aberrant nuclear reassembly
Catastrophic one-cell arrest
Excessive blebbing
Multiple nuclei in 1, 2, or 4-cell embryos
Osmotically/ pressure sensitive
P0 spindle positioning defect
Pronuclei fail to migrate
Slow cell cycle
Synchronous second division
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_7D6 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_F54B3.3 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of F54B3.3 Browse using GBrowse
Gene Graph