RNAi Experiment: PF:GL1_8B5 |
Ace View of PF:GL1_8B5
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Genes Inhibited |
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Gene Inhibited |
C32F10.2 (lin-35) |
Description of "C32F10.2" |
lin-35 encodes the C. elegans retinoblastoma protein (Rb) ortholog; lin-35 was first identified in screens for synthetic multivulva (synMuv) genes and as a class B synMuv gene, functions redundantly with class A genes to antagonize Ras signaling and negatively regulate vulval development; in addition, lin-35 activity is required redundantly with: 1) pha-1 and ubc-18 for early steps in pharyngeal morphogenesis, 2) fzr-1 for normal patterns of postembryonic proliferation, 3) xnp-1 for somatic gonad development, and 4) psa-1 for fertility and embryonic and larval development; on its own, lin-35 is also required for wild-type levels of fertility; LIN-35 is expressed broadly in embryos and L1 larvae, but in later larvae and adults is detected in vulval precursor cells and their descendants as well as a subset of head and tail cells. |
KOG Annotation  |
Rb (Retinoblastoma tumor suppressor)-related protein
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Evidence for Inhibition |
ePCR |
Status: Unique_ePCR (I:5813223..5814364)
Transcript overlap: 886 nt |
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Sliding Window |
Raw score: 802
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene) |
Wormbase (BLAST) |
RNAi primary |
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External "C32F10.2" Links |
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PF:GL1_8B5 Phenotypes |
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Adult |
Rup
Observed in <10% of progeny.
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PF:GL1_8B5 Experiment Details |
Ace View |
Laboratory |
KK |
RNAi Reagent & Type |
sjj_C32F10.2 (PCR_reagent) |
Remark |
PCR product contains flanking T7 promoter sequences |
Reference |
Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" |
Delivery Method |
Injection |
Date |
2002-11-19 |
Gene Plot of C32F10.2 |
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