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RNAi Experiment: PF:GL1_8B9 Ace View of PF:GL1_8B9
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C38C10.4 (gpr-2)
Description of "C38C10.4"  gpr-2 encodes a protein containing a GPR (G Protein Regulator)/GoLoco motif characteristic of guanine nucleotide exchange factors specific for G-alpha GTPases; GPR-2 appears to function redundantly during early embryogenesis and germ-line development to regulate chromosome and spindle movements during cell division; GPR-2 likely acts as a positive regulator of G protein signaling and specifically, may regulate GOA-1 signaling in the embryo; GPR-2 forms a protein complex with the nearly identical GPR-1 and with LIN-5, a coiled-coil protein that is required for proper localization of GPR-2 to the cell cortex and spindle asters of the early embryo; in addition, proper GPR-2/GPR-1/LIN-5 localization between the P2 and EMS blastomeres at the four-cell stage, which may contribute to spindle positioning in EMS, requires the MES-1/SRC-1 tyrosine kinase signaling pathway; GPR-2 is believed to act downstream of, or in parallel to, PAR-3, a PDZ domain-containing protein, in mitotic spindle positioning in the early embryo.
Evidence for Inhibition 
ePCR Status: Best_ePCR (III:9391789..9392950)
Transcript overlap: 1059 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 996
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.59
Wormbase (BLAST) RNAi primary
External "C38C10.4" Links  Wormbase NCBI
Gene Inhibited  F22B7.13 (gpr-1)
Description of "F22B7.13"  Associated GO Term : GTPase activator activity
Evidence for Inhibition 
ePCR Status: Best_ePCR (III:8628947..8630106)
Transcript overlap: 1059 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 692
Relative score: 0.695 (Best raw score= for Gene )
Specificity index: 0.41
Wormbase (BLAST) RNAi primary
External "F22B7.13" Links  Wormbase NCBI
PF:GL1_8B9 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 %
Adult  Him >0.4% male progeny observed.
Phenotype  ABa, ABp and EMS divide synchronously
Aberrant AB or P1 spindle orientation
Division axis of ABa or ABp aberrant
Multiple nuclei in 1, 2, or 4-cell embryos
Nuclei reform next to cell division remnant
P0 spindle positioning defect
P1 aster abnormal
Pronuclei meet more centrally
Synchronous second division
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_8B9 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_F22B7.5 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of C38C10.4 Browse using GBrowse
Gene Graph
Gene Plot of F22B7.13 Browse using GBrowse
Gene Graph