Ace View of PF:GL1_8B9 | |||
Gene Inhibited | C38C10.4 (gpr-2) | ||
Description of "C38C10.4" | gpr-2 encodes a protein containing a GPR (G Protein Regulator)/GoLoco motif characteristic of guanine nucleotide exchange factors specific for G-alpha GTPases; GPR-2 appears to function redundantly during early embryogenesis and germ-line development to regulate chromosome and spindle movements during cell division; GPR-2 likely acts as a positive regulator of G protein signaling and specifically, may regulate GOA-1 signaling in the embryo; GPR-2 forms a protein complex with the nearly identical GPR-1 and with LIN-5, a coiled-coil protein that is required for proper localization of GPR-2 to the cell cortex and spindle asters of the early embryo; in addition, proper GPR-2/GPR-1/LIN-5 localization between the P2 and EMS blastomeres at the four-cell stage, which may contribute to spindle positioning in EMS, requires the MES-1/SRC-1 tyrosine kinase signaling pathway; GPR-2 is believed to act downstream of, or in parallel to, PAR-3, a PDZ domain-containing protein, in mitotic spindle positioning in the early embryo. | ||
Evidence for Inhibition | |||
External "C38C10.4" Links | ![]() ![]() |
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Gene Inhibited | F22B7.13 (gpr-1) | ||
Description of "F22B7.13" | Associated GO Term : GTPase activator activity | ||
Evidence for Inhibition | |||
External "F22B7.13" Links | ![]() ![]() |
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Embryonic | Emb Penetrance 80 - 100 % | ||
Adult | Him
>0.4% male progeny observed. |
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Phenotype | ABa, ABp and EMS divide synchronously Aberrant AB or P1 spindle orientation Division axis of ABa or ABp aberrant Multiple nuclei in 1, 2, or 4-cell embryos Nuclei reform next to cell division remnant P0 spindle positioning defect P1 aster abnormal Pronuclei meet more centrally Synchronous second division |
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Movies/Stacks/Images | ![]() ![]() ![]() |
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Laboratory | KK | ||
RNAi Reagent & Type | sjj_F22B7.5 (PCR_reagent) | ||
Remark | PCR product contains flanking T7 promoter sequences | ||
Reference | Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans" | ||
Delivery Method | Injection | ||
Date | 2002-11-19 | ||
Gene Plot of C38C10.4 | |||
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Gene Plot of F22B7.13 | |||
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