Link to H02I12.8RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentLink to Y17G9B.3Link to Y62E10A.15geneMap Link to Y17G9B.3RNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentLink to H02I12.8Link to Y62E10A.15geneMap RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentLink to H02I12.8Link to Y17G9B.3Link to C01F6.3geneMap Link to C01F6.2RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentLink to H02I12.8Link to Y17G9B.3geneMap
Go to RNAiDB Home Adult Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie
 
RNAi Experiment: PF:GL1_8D2 Ace View of PF:GL1_8D2
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  H02I12.8 (cyp-31A2)
Description of "H02I12.8"  Associated GO Term : monooxygenase activity
KOG Annotation  Cytochrome P450 CYP4/CYP19/CYP26 subfamilies
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (IV:11403949..11405074)
Transcript overlap: 943 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 817
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.705
Wormbase (BLAST) RNAi primary
External "H02I12.8" Links  Wormbase NCBI
Gene Inhibited  Y17G9B.3 (cyp-31A3)
Description of "Y17G9B.3"  The Y17G9B.3 gene encodes a homolog of the human gene CYP7B1, which when mutated leads to pseudovitamin D deficiency rickets (OMIM:264700).
KOG Annotation  Cytochrome P450 CYP4/CYP19/CYP26 subfamilies
What's a KOG?
Evidence for Inhibition 
Sliding Window Raw score: 90
Relative score: 0.11 (Best raw score= for Gene )
Specificity index: 0.078
Wormbase (BLAST) RNAi secondary
External "Y17G9B.3" Links  Wormbase NCBI
Gene Inhibited  Y62E10A.15 (cyp-31A5)
Evidence for Inhibition 
Sliding Window Raw score: 114
Relative score: 0.14 (Best raw score= for Gene )
Specificity index: 0.098
Wormbase (BLAST) RNAi primary
External "Y62E10A.15" Links  Wormbase NCBI
Gene Inhibited  C01F6.3 (cyp-31A1)
Evidence for Inhibition 
Sliding Window Raw score: 138
Relative score: 0.169 (Best raw score= for Gene )
Specificity index: 0.119
Wormbase (BLAST) RNAi secondary
External "C01F6.3" Links  Wormbase NCBI
PF:GL1_8D2 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 80 - 100 %
Phenotype  AB aster resembles P1 aster
ABa, ABp and EMS divide synchronously
Aberrant AB or P1 spindle orientation
Delayed P0 spindle rotation
Division axis of ABa or ABp aberrant
EMS fails to extend anteriorly
Multiple nuclei in 1, 2, or 4-cell embryos
Osmotically/ pressure sensitive
P0 spindle positioning defect
P2 and EMS divide synchronously
Polar body reabsorbed
Pseudocleavage absent
Synchronous second division
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_8D2 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_H02I12.8 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of H02I12.8 Browse using GBrowse
Gene Graph
Gene Plot of Y17G9B.3 Browse using GBrowse
Gene Graph
Gene Plot of Y62E10A.15 Browse using GBrowse
Gene Graph
Gene Plot of C01F6.3 Browse using GBrowse
Gene Graph