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RNAi Experiment: PF:GL1_8E5 Ace View of PF:GL1_8E5
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  R06F6.1 (cdl-1)
Description of "R06F6.1"  cdl-1 encodes a homolog of human hairpin (stem-loop) binding proteins (HBP/SLBP) that bind to the hairpin (stem-loop) structure in the 3' untranslated region (3' UTR) of histone mRNAs, and thus promote histone pre-mRNA processing and translation of mature histone mRNA; CDL-1 is required for normally high levels of histone gene expression, normal cell division during late larval development, embryonic viability, normal vulval morphogenesis, normally rapid apoptosis, and fertility; CDL-1 binds to the stem-loop structure in the 3' UTR of core-histone mRNA; the cdl-1 promoter is most active in dividing cells during embryogenesis and postembryonic development; both CDL-1 and human HBP contain a minimal RNA-binding domain (RBD) of roughly 73 amino acids that has no similarity with other known RNA-binding motifs.
KOG Annotation  Histone mRNA stem-loop binding protein
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (II:10785639..10786621)
Transcript overlap: 821 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 741
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "R06F6.1" Links  Wormbase NCBI
PF:GL1_8E5 Phenotypes Run Phenoblast! Run Phenoblast Help with Phenotypes
Embryonic  Emb Penetrance 100 %
Phenotype  Delayed P0 spindle rotation
Ectopic cleavage furrows
EMS extends too far anteriorly
Excessive blebbing
Nuclei reform next to cell division remnant
P0 spindle positioning defect
Movies/Stacks/Images  /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif /static/images/movie.gif Help
PF:GL1_8E5 Experiment Details Ace View
Laboratory  KK
RNAi Reagent & Type  sjj_R06F6.1 (PCR_reagent)
Remark  PCR product contains flanking T7 promoter sequences
Reference  Piano et al. (2002) Curr Biol "Gene Clustering Based on RNAi Phenotypes of Ovary-Enriched Genes in C. elegans"
Delivery Method  Injection
Date  2002-11-19
Gene Plot of R06F6.1 Browse using GBrowse
Gene Graph