Link to B0035.8RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentLink to ZK131.5geneMap Link to F54E12.4Link to F54E12.5RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentgeneMap Link to F08G2.1Link to F08G2.2RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentgeneMap Link to ZK131.5RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentLink to B0035.8geneMap Link to H02I12.6Link to H02I12.7RNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentgeneMap Link to F55G1.3RNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentgeneMap
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RNAi Experiment: Simmer:B0035.8 Ace View of Simmer:B0035.8
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  B0035.8 (his-48)
Description of "B0035.8"  his-48 encodes an H2B histone; by homology, HIS-48 is predicted to function as a nucleosome component required for packaging of DNA into chromatin; his-48 is a replication-dependent histone locus that resides in a histone gene-rich region on chromosome IV.
Evidence for Inhibition 
ePCR Status: Best_ePCR (IV:11324571..11325351)
Transcript overlap: 411 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 368
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.276
Wormbase (BLAST) RNAi primary
External "B0035.8" Links  Wormbase NCBI
Gene Inhibited  F54E12.4 (his-58)
Description of "F54E12.4"  his-58 encodes an H2B histone.
Evidence for Inhibition 
ePCR Status: Top85_percentile_ePCR (IV:11339442..11340222)
Transcript overlap: 372 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 330
Relative score: 0.897 (Best raw score= for Gene )
Specificity index: 0.247
Wormbase (BLAST) RNAi primary
External "F54E12.4" Links  Wormbase NCBI
Gene Inhibited  F08G2.1 (his-44)
Description of "F08G2.1"  his-44 encodes an H2B histone.
Evidence for Inhibition 
Sliding Window Raw score: 86
Relative score: 0.234 (Best raw score= for Gene )
Specificity index: 0.064
Wormbase (BLAST) RNAi secondary
External "F08G2.1" Links  Wormbase NCBI
Gene Inhibited  ZK131.5 (his-11)
Description of "ZK131.5"  his-11 encodes an H2B histone; by homology, HIS-11 is predicted to function as a nucleosome component required for packaging of DNA into chromatin; his-11 is a replication-dependent histone locus that resides in the HIS3 cluster on chromosome II.
Evidence for Inhibition 
Sliding Window Raw score: 82
Relative score: 0.223 (Best raw score= for Gene )
Specificity index: 0.061
Wormbase (BLAST) RNAi secondary
External "ZK131.5" Links  Wormbase NCBI
Gene Inhibited  H02I12.6 (his-66)
Description of "H02I12.6"  his-66 encodes an H2B histone.
Evidence for Inhibition 
Sliding Window Raw score: 351
Relative score: 0.954 (Best raw score= for Gene )
Specificity index: 0.263
Wormbase (BLAST) RNAi primary
External "H02I12.6" Links  Wormbase NCBI
Gene Inhibited  F55G1.3 (his-62)
Description of "F55G1.3"  his-62 encodes an H2B histone.
KOG Annotation  Histone H2B
What's a KOG?
Evidence for Inhibition 
Sliding Window Raw score: 118
Relative score: 0.321 (Best raw score= for Gene )
Specificity index: 0.088
Wormbase (BLAST) RNAi secondary
External "F55G1.3" Links  Wormbase NCBI
Simmer:B0035.8 Phenotypes Help with Phenotypes
Adult  Ste
Simmer:B0035.8 Experiment Details Ace View
Laboratory  NL
RNAi Reagent & Type  sjj_B0035.8 (PCR_reagent)
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Date  2003-10-01
Gene Plot of F54E12.4 Browse using GBrowse
Gene Graph
Gene Plot of F08G2.1 Browse using GBrowse
Gene Graph
Gene Plot of ZK131.5 Browse using GBrowse
Gene Graph
Gene Plot of H02I12.6 Browse using GBrowse
Gene Graph
Gene Plot of F55G1.3 Browse using GBrowse
Gene Graph
Gene Plot of B0035.8 Browse using GBrowse
Gene Graph