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RNAi Experiment: Simmer:B0547.1 Ace View of Simmer:B0547.1
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  B0547.1 (csn-5)
Description of "B0547.1"  csn-5 encodes an ortholog of subunit 5 of the COP9 signalosome complex (CSN5), which in turn is a pan-eukaryotic multiprotein complex that positively regulates E3 ubiquitin ligases, physically interacts with SCF-type E3 ubiquitin ligases, and probably regulates protein degradation; CSN-5 (along with KGB-1 and ZYX-1) physically binds germline RNA helicases (GLH proteins), and csn-5(RNAi) results in sterile worms with small gonads and no oocytes, causing oogenesis to cease, but not affecting the initial assembly of P granules -- a defect essentially identical to that produced by RNAi with a combination of glh-1 and glh-4.
KOG Annotation  COP9 signalosome, subunit CSN5
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (IV:5649987..5651016)
Transcript overlap: 784 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 742
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "B0547.1" Links  Wormbase NCBI
Simmer:B0547.1 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 50 - 80 %
Simmer:B0547.1 Experiment Details Ace View
Laboratory  NL
RNAi Reagent & Type  sjj_B0547.1 (PCR_reagent)
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Date  2003-10-01
Gene Plot of B0547.1 Browse using GBrowse
Gene Graph