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RNAi Experiment: Simmer:C50F4.11 Ace View of Simmer:C50F4.11
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C50F4.11 (mdf-1)
Description of "C50F4.11"  mdf-1 encodes a coiled-coil domain-containing protein that is orthologous to the Saccharomyces cerevisiae mitotic spindle checkpoint protein Mad1p; consistent with a role as a checkpoint protein, MDF-1 is required in C. elegans for mitotic genome stability and hence, long-term survival and fertility; in vitro, MDF-1 interacts with MDF-2, the C. elegans Mad2p ortholog, and with IFY-1, a Securin homolog; the maternal effect lethality produced by an mdf-1 mutation can be suppressed by a mutation in fzy-1, which encodes the C. elegans Cdc20 homolog.
Evidence for Inhibition 
ePCR Status: Unique_ePCR (V:9546026..9546960)
Transcript overlap: 735 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 693
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "C50F4.11" Links  Wormbase NCBI
Simmer:C50F4.11 Phenotypes Help with Phenotypes
Adult  Pvl
Unc
Simmer:C50F4.11 Experiment Details Ace View
Laboratory  NL
RNAi Reagent & Type  sjj_C50F4.11 (PCR_reagent)
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Date  2003-10-01
Gene Plot of C50F4.11 Browse using GBrowse
Gene Graph