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RNAi Experiment: Simmer:F13B10.2 Ace View of Simmer:F13B10.2
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F13B10.2 (rpl-3)
Description of "F13B10.2"  rpl-3 encodes a large ribosomal subunit L3 protein required in mass RNAi assays for embryonic and larval viability, fertility, and general health; the rpl-3 transcription unit has a nonsense transcript that is up-regulated in vivo by smg[-] mutations, indicating that rpl-3 is a natural substrate for SMG-mediated nonsense suppresssion; several other natural mRNA substrates of SMG suppression (e.g., rpl-7, rpl-10, rpl-12) have protein products that are involved in translation.
Evidence for Inhibition 
ePCR Status: Unique_ePCR (III:3868388..3869555)
Transcript overlap: 880 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 817
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "F13B10.2" Links  Wormbase NCBI
Simmer:F13B10.2 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 50 - 80 %
Larval  Lva
Adult  Ste brood size (1 out of 5)
Simmer:F13B10.2 Experiment Details Ace View
Laboratory  NL
RNAi Reagent & Type  sjj_F13B10.2 (PCR_reagent)
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Date  2003-10-01
Gene Plot of F13B10.2 Browse using GBrowse
Gene Graph