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RNAi Experiment: Simmer:F18H3.5:Repeat_a Ace View of Simmer:F18H3.5:Repeat_a
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F18H3.5 (cdk-4)
Description of "F18H3.5"  cdk-4 encodes two isoforms of a cyclin-dependent serine/threonine protein kinase orthologous to human CDK4 and CDK6 (OMIM:123829 and OMIM:603368, mutated in cutanous malignant melanoma) which complex with D-type cyclins to regulate progression through the G1 phase of the cell cycle; CDK-4 activity is essential for G1 progression in postembryonic blast cells and as a result, cdk-4 mutant animals generally arrest during larval stages; the lethality generated by cdk mutations, also seen in animals doubly mutant for cdk-4 and cyd-1, a C. elegans D-type cyclin, can be suppressed by mutations in lin-35/Rb suggesting that, as in other organisms, LIN-35/Rb may be a major target of CDK-4/CYD-1 kinase activity; CDK-4 expression is first detected in neuronal and hypodermal lineages during mid-to-late embryogenesis, with postembryonic expression detected in hypodermal seam cells, cells of the P lineage which will give rise to ventral cord neurons, and cells of the somatic gonad, the vulva, and the intestine.
KOG Annotation  Protein kinase PCTAIRE and related kinases
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (X:13516067..13517255)
Transcript overlap: 791 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 707
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "F18H3.5" Links  Wormbase NCBI
Simmer:F18H3.5:Repeat_a Phenotypes Help with Phenotypes
Adult  Dpy
Pvl
Rup
Unc
Simmer:F18H3.5:Repeat_a Experiment Details Ace View
Laboratory  NL
RNAi Reagent & Type  sjj_F18H3.5 (PCR_reagent)
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Date  2003-10-01
Gene Plot of F18H3.5 Browse using GBrowse
Gene Graph