RNAi Card Page

RNAi Experiment: Simmer:F18H3.5:Repeat_a

Genes Inhibited

Gene Inhibited

Description of "F18H3.5"

cdk-4 encodes two isoforms of a cyclin-dependent serine/threonine protein kinase orthologous to human CDK4 and CDK6 (OMIM:123829 and OMIM:603368, mutated in cutanous malignant melanoma) which complex with D-type cyclins to regulate progression through the G1 phase of the cell cycle; CDK-4 activity is essential for G1 progression in postembryonic blast cells and as a result, cdk-4 mutant animals generally arrest during larval stages; the lethality generated by cdk mutations, also seen in animals doubly mutant for cdk-4 and cyd-1, a C. elegans D-type cyclin, can be suppressed by mutations in lin-35/Rb suggesting that, as in other organisms, LIN-35/Rb may be a major target of CDK-4/CYD-1 kinase activity; CDK-4 expression is first detected in neuronal and hypodermal lineages during mid-to-late embryogenesis, with postembryonic expression detected in hypodermal seam cells, cells of the P lineage which will give rise to ventral cord neurons, and cells of the somatic gonad, the vulva, and the intestine.

KOG Annotation

Protein kinase PCTAIRE and related kinases

Evidence for Inhibition

ePCR	Status: Unique_ePCR (X:13516067..13517255) Transcript overlap: 791 nt
Sliding Window	Raw score: 707 Relative score: 1 (This gene has the best score for this RNAi reagent) Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST)	RNAi primary