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RNAi Experiment: Simmer:F21H12.5:Screen Ace View of Simmer:F21H12.5:Screen
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F21H12.5 (fbf-2)
Description of "F21H12.5"  fbf-2 encodes an RNA-binding protein that is one of 11 C. elegans members of the PUF family (Pumilio and FBF) of translational regulators; FBF-2 is nearly identical to FBF-1 with which it is largely redundant in regulating two aspects of germline development: 1)maintenance of stem cell proliferation, and 2)the hermaphroditic switch between spermatogenesis and oogenesis; in maintaining germline stem cells, the FBF proteins, acting through NOS-3, negatively regulate the activity of gld-1 mRNA, which encodes a translational repressor required for meiotic entry; in regulating the sperm-to-oocyte switch, the FBFs act downstream of GLD-3 to negatively regulate the activity of fem-3 mRNA, which encodes a novel protein required for germline sex determination; consistent with their role in germline development, FBF-1 and FBF-2 are expressed in the germline cytoplasm, becoming enriched in the mitotic region during the L4 larval and adult stages.
KOG Annotation  Translational repressor Pumilio/PUF3 and related RNA-binding proteins (Puf superfamily)
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (II:6089095..6090283)
Transcript overlap: 1048 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 964
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.733
Wormbase (BLAST) RNAi primary
External "F21H12.5" Links  Wormbase NCBI
Gene Inhibited  H12I13.4 (fbf-1)
Description of "H12I13.4"  fbf-1 encodes an RNA-binding protein that is one of 11 C. elegans members of the PUF family (Pumilio and FBF) of translational regulators; FBF-1 is nearly identical to FBF-2 with which it is largely redundant in regulating two aspects of germline development: 1)maintenance of stem cell proliferation, and 2)the hermaphroditic switch between spermatogenesis and oogenesis; in maintaining germline stem cells, the FBF proteins, acting through NOS-3, negatively regulate the activity of gld-1 mRNA, which encodes a translational repressor required for meiotic entry; in regulating the sperm-to-oocyte switch, the FBFs act downstream of GLD-3 to negatively regulate the activity of fem-3 mRNA, which encodes a novel protein required for germline sex determination; consistent with their role in germline development, FBF-1 and FBF-2 are expressed in the germline cytoplasm, becoming enriched in the mitotic region during the L4 larval and adult stages.
KOG Annotation  Translational repressor Pumilio/PUF3 and related RNA-binding proteins (Puf superfamily)
What's a KOG?
Evidence for Inhibition 
Sliding Window Raw score: 351
Relative score: 0.364 (Best raw score= for Gene )
Specificity index: 0.267
Wormbase (BLAST) RNAi primary
External "H12I13.4" Links  Wormbase NCBI
Simmer:F21H12.5:Screen Phenotypes Help with Phenotypes
Adult  Stp
Simmer:F21H12.5:Screen Experiment Details Ace View
Laboratory  NL
RNAi Reagent & Type  sjj_F21H12.5 (PCR_reagent)
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Date  2003-10-01
Gene Plot of H12I13.4 Browse using GBrowse
Gene Graph
Gene Plot of F21H12.5 Browse using GBrowse
Gene Graph