| RNAi Experiment: Simmer:F45F2.13 |
Ace View of Simmer:F45F2.13
|
| Genes Inhibited |
 |
| Gene Inhibited |
F45F2.13 (his-6) |
| Description of "F45F2.13" |
his-6 encodes an H3 histone. |
| Evidence for Inhibition |
| ePCR |
Status: Unique_ePCR (V:8536762..8537680)
Transcript overlap: 411 nt |
|
| Sliding Window |
Raw score: 369
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.204 |
| Wormbase (BLAST) |
RNAi primary |
|
| External "F45F2.13" Links |
  |
| Gene Inhibited |
K03A1.1 (his-40) |
| Description of "K03A1.1" |
his-40 is a predicted pseudogene whose partial translation products, in a given reading frame, display strong similarity to histones; large-scale RNAi screens targeting his-40 sequences in the hypersensitive rrf-3 background do result in embryonic and larval lethality, indicating that his-40 either has a real biological function or that there is cross-reactivity with mRNA from other histone genes in these experiments. |
| Evidence for Inhibition |
| Sliding Window |
Raw score: 147
Relative score: 0.398 (Best raw score= for Gene )
Specificity index: 0.081 |
| Wormbase (BLAST) |
RNAi secondary |
|
| External "K03A1.1" Links |
|
| Gene Inhibited |
F17E9.10 (his-32) |
| Description of "F17E9.10" |
his-32 encodes an H3 histone; by homology, HIS-32 is predicted to function as a nucleosome component required for packaging of DNA into chromatin; his-32 is a replication-dependent histone locus that resides in the HIS5 cluster on chromosome IV. |
| Evidence for Inhibition |
| Sliding Window |
Raw score: 61
Relative score: 0.165 (Best raw score= for Gene )
Specificity index: 0.034 |
| Wormbase (BLAST) |
RNAi secondary |
|
| External "F17E9.10" Links |
|
| Gene Inhibited |
K06C4.13 (his-27) |
| Description of "K06C4.13" |
his-27 encodes an H3 histone; by homology, HIS-27 is predicted to function as a nucleosome component required for packaging of DNA into chromatin; his-27 is a replication-dependent histone locus that resides in the HIS4 cluster on chromosome V. |
| Evidence for Inhibition |
| Sliding Window |
Raw score: 302
Specificity index: 0.167 |
| Wormbase (BLAST) |
RNAi primary |
|
| External "K06C4.13" Links |
|
| Gene Inhibited |
T10C6.13 (his-2) |
| Description of "T10C6.13" |
his-2 encodes an H3 histone; his-2 is contained within the histone gene cluster HIS1. |
| Evidence for Inhibition |
| Sliding Window |
Raw score: 213
Relative score: 0.577 (Best raw score= for Gene )
Specificity index: 0.118 |
| Wormbase (BLAST) |
RNAi secondary |
|
| External "T10C6.13" Links |
|
| Gene Inhibited |
F55G1.2 (his-59) |
| Description of "F55G1.2" |
his-59 encodes an H3 histone; by homology, HIS-59 is predicted to function as a nucleosome component required for packaging of DNA into chromatin; his-59 is a replication-dependent histone locus that resides in a histone gene-rich region on chromosome IV. |
| KOG Annotation |
Histones H3 and H4
|
|
| Evidence for Inhibition |
| Sliding Window |
Raw score: 89
Relative score: 0.241 (Best raw score= for Gene )
Specificity index: 0.049 |
| Wormbase (BLAST) |
RNAi secondary |
|
| External "F55G1.2" Links |
|
| Gene Inhibited |
F07B7.5 (his-49) |
| Description of "F07B7.5" |
his-49 encodes an H3 histone; by homology, HIS-49 is predicted to function as a nucleosome component required for packaging of DNA into chromatin; his-49 is a replication-dependent histone locus that resides in a histone gene-rich region on chromosome V. |
| Evidence for Inhibition |
| Sliding Window |
Raw score: 302
Relative score: 0.818 (Best raw score= for Gene )
Specificity index: 0.167 |
| Wormbase (BLAST) |
RNAi primary |
|
| External "F07B7.5" Links |
|
| Gene Inhibited |
K06C4.5 (his-17) |
| Description of "K06C4.5" |
his-17 encodes an H3 histone; his-17 is contained within the histone gene cluster HIS4. |
| Evidence for Inhibition |
| Sliding Window |
Raw score: 322
Relative score: 0.873 (Best raw score= for Gene )
Specificity index: 0.178 |
| Wormbase (BLAST) |
RNAi primary |
|
| External "K06C4.5" Links |
|
| Simmer:F45F2.13 Phenotypes |
|
|
| Embryonic |
Emb Penetrance 50 - 80 % |
| Larval |
Lva
|
| Adult |
Unc
|
| Simmer:F45F2.13 Experiment Details |
Ace View |
| Laboratory |
NL |
| RNAi Reagent & Type |
sjj_F45F2.13 (PCR_reagent) |
| Strain |
NL4256 rrf-3(pk1426) |
| Reference |
Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." |
| Date |
2003-10-01 |
| Gene Plot of T10C6.13 |
Browse using GBrowse
|
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| Gene Plot of F55G1.2 |
Browse using GBrowse
|
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| Gene Plot of F17E9.10 |
Browse using GBrowse
|
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| Gene Plot of K03A1.1 |
Browse using GBrowse
|
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| Gene Plot of F45F2.13 |
Browse using GBrowse
|
 |
| Gene Plot of F07B7.5 |
Browse using GBrowse
|
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| Gene Plot of K06C4.13 |
Browse using GBrowse
|
 |
| Gene Plot of K06C4.5 |
Browse using GBrowse
|
 |
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