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RNAi Experiment: Simmer:F55H2.4 Ace View of Simmer:F55H2.4
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  K08E7.3 (let-99)
Description of "K08E7.3"  let-99 encodes a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); LET-99 has no obvious homologs in non-nematodes, but has a truncated paralog (LRG-1) in C. elegans; LET-99 is required for the mitotic spindle to be properly oriented with respect to the axis of cellular polarity, both during anterior migration and rotation of the nuclear-centrosome complex and during anaphase; let-99 mutants exhibit hyperactive nuclear movement and abnormal anaphase spindle pole behavior; LET-99 is mislocalized in par-2 and par-3 mutants; like other proteins containing DEP domains, LET-99 may act as part of a G protein signalling pathway (e.g., the one controlling spindle position).
Evidence for Inhibition 
Sliding Window Raw score: 199
Relative score: 0.177 (Best raw score= for Gene )
Specificity index: 0.15
Wormbase (BLAST) RNAi secondary
External "K08E7.3" Links  Wormbase NCBI
Gene Inhibited  F55H2.4 (lrg-1)
Description of "F55H2.4"  lrg-1 encodes a truncated paralog of let-99, a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); lrg-1(RNAi) animals have no obvious phenotype; since the nucleotide similarity of lrg-1 to let-99 extends beyond the lrg-1 CDS, and since lrg-1 has no obvious function in RNAi assays, this gene may be decaying towards pseudogene status.
Evidence for Inhibition 
Sliding Window Raw score: 1124
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.85
Wormbase (BLAST) RNAi primary
External "F55H2.4" Links  Wormbase NCBI
Simmer:F55H2.4 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 90 %
Simmer:F55H2.4 Experiment Details Ace View
Laboratory  NL
RNAi Reagent & Type  sjj_F55H2.4 (PCR_reagent)
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Date  2003-10-01
Gene Plot of K08E7.3 Browse using GBrowse
Gene Graph
Gene Plot of F55H2.4 Browse using GBrowse
Gene Graph