RNAi Experiment: Simmer:M7.1 |
Ace View of Simmer:M7.1
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Genes Inhibited |
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Gene Inhibited |
M7.1 (let-70) |
Description of "M7.1" |
let-70 encodes a class I E2 ubiquitin conjugating enzyme; let-70 was identified in screens for essential genes and phenotypic analyses indicate that loss of let-70 activity results in embryonic and larval lethality with defects in sarcomere assembly; purified LET-70 can stimulate the self-ubiquitylation activities of CHN-1 and UFD-2, E4 ubiquitin conjugation factors and, in addition, can stimulate the CHN-1- and UFD-2-dependent multiubiquitylation of UNC-45, a myosin-directed chaperone; in this manner, LET-70 may regulate UNC-45 degradation and myosin assembly; RNA blot analyses and a let-70-lacZ reporter fusion reveal that let-70 is abundantly expressed at all stages of development, including the dauer larval stage; strong staining is observed in most somatic tissues until adult stages, when expression generally becomes restricted to the nervous system. |
KOG Annotation  |
Ubiquitin-protein ligase
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Evidence for Inhibition |
ePCR |
Status: Unique_ePCR (IV:11081430..11082308)
Transcript overlap: 610 nt |
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Sliding Window |
Raw score: 505
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.847 |
Wormbase (BLAST) |
RNAi primary |
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External "M7.1" Links |
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Gene Inhibited |
F52C6.12 |
Description of "F52C6.12" |
Associated GO Term : ubiquitin-like activating enzyme activity |
Evidence for Inhibition |
Sliding Window |
Raw score: 91
Relative score: 0.18 (Best raw score= for Gene )
Specificity index: 0.153 |
Wormbase (BLAST) |
RNAi primary |
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External "F52C6.12" Links |
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Simmer:M7.1 Phenotypes |
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Embryonic |
Emb Penetrance 100 % |
Simmer:M7.1 Experiment Details |
Ace View |
Laboratory |
NL |
RNAi Reagent & Type |
sjj_M7.1 (PCR_reagent) |
Strain |
NL4256 rrf-3(pk1426) |
Reference |
Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." |
Date |
2003-10-01 |
Gene Plot of F52C6.12 |
Browse using GBrowse
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Gene Plot of M7.1 |
Browse using GBrowse
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