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RNAi Experiment: Simmer:R09B3.4:Screen_A Ace View of Simmer:R09B3.4:Screen_A
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  R09B3.4 (ubc-12)
Description of "R09B3.4"  The ubc-12 gene encodes a ubiquitin-conjugating enzyme whose substrate is NED-8, and which is required for both embryogenesis and terminal hypodermal differentiation; ubc-12(RNAi) mutants either arrest during embryonic development or show pleotropic defects (e.g., vulval eversion during the L4 stage), as well as defective alae.
KOG Annotation  Ubiquitin-protein ligase
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (I:11911188..11911966)
Transcript overlap: 606 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 564
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "R09B3.4" Links  Wormbase NCBI
Simmer:R09B3.4:Screen_A Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 20 - 40 %
Larval  Gro
Adult  Pvl
Phenotype  Unclassified Thin
Simmer:R09B3.4:Screen_A Experiment Details Ace View
Laboratory  NL
RNAi Reagent & Type  sjj_R09B3.4 (PCR_reagent)
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Date  2003-10-01
Gene Plot of R09B3.4 Browse using GBrowse
Gene Graph