RNAi Experiment: Simmer:R119.6:Screen_A |
Ace View of Simmer:R119.6:Screen_A
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Genes Inhibited |
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Gene Inhibited |
R119.6 (taf-4) |
Description of "R119.6" |
taf-4 encodes a TAFII (TBP(TATA-binding protein)-associated factor) that is orthologous to human TAF4 (hTAFII130); TAF-4 is predicted to be a component of the TFIID mRNA transcription complex and is generally required for early embryonic mRNA transcription; consistent with this observation, taf-4(RNAi) results in embryonic lethality at the ~100-cell stage with little cell differentiation, similar to the loss of AMA-1/RNA polymerase II activity; TAF-4 is detected in nuclei in the adult germline, oocytes, and embryo, from the 2-cell stage through early morphogenesis. |
KOG Annotation  |
TATA box binding protein (TBP)-associated factor, RNA polymerase II
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Evidence for Inhibition |
ePCR |
Status: Best_ePCR (I:384765..386429)
Transcript overlap: 725 nt |
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Sliding Window |
Raw score: 683
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.876 |
Wormbase (BLAST) |
RNAi primary |
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External "R119.6" Links |
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Gene Inhibited |
R119.5 |
Description of "R119.5" |
Associated GO Term : protein-L-isoaspartate (D-aspartate) O-methyltransferase activity |
KOG Annotation  |
Protein-L-isoaspartate(D-aspartate) O-methyltransferase
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Evidence for Inhibition |
ePCR |
Status: Bottom50_percentile_ePCR (I:384765..386429)
Transcript overlap: 97 nt |
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Sliding Window |
Raw score: 97
Relative score: 0.142 (Best raw score= for Gene )
Specificity index: 0.124 |
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External "R119.5" Links |
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Simmer:R119.6:Screen_A Phenotypes |
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Embryonic |
Emb Penetrance 100 % |
Larval |
Gro
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Adult |
Ste
brood size (1 out of 5) Unc
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Simmer:R119.6:Screen_A Experiment Details |
Ace View |
Laboratory |
NL |
RNAi Reagent & Type |
sjj_R119.6 (PCR_reagent) |
Strain |
NL4256 rrf-3(pk1426) |
Reference |
Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." |
Date |
2003-10-01 |
Gene Plot of R119.5 |
Browse using GBrowse
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Gene Plot of R119.6 |
Browse using GBrowse
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