Link to R119.6RNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentgeneMap Link to R119.6Link to R119.5RNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentgeneMap
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RNAi Experiment: Simmer:R119.6:Screen_A Ace View of Simmer:R119.6:Screen_A
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  R119.6 (taf-4)
Description of "R119.6"  taf-4 encodes a TAFII (TBP(TATA-binding protein)-associated factor) that is orthologous to human TAF4 (hTAFII130); TAF-4 is predicted to be a component of the TFIID mRNA transcription complex and is generally required for early embryonic mRNA transcription; consistent with this observation, taf-4(RNAi) results in embryonic lethality at the ~100-cell stage with little cell differentiation, similar to the loss of AMA-1/RNA polymerase II activity; TAF-4 is detected in nuclei in the adult germline, oocytes, and embryo, from the 2-cell stage through early morphogenesis.
KOG Annotation  TATA box binding protein (TBP)-associated factor, RNA polymerase II
What's a KOG?
Evidence for Inhibition 
ePCR Status: Best_ePCR (I:384765..386429)
Transcript overlap: 725 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 683
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.876
Wormbase (BLAST) RNAi primary
External "R119.6" Links  Wormbase NCBI
Gene Inhibited  R119.5
Description of "R119.5"  Associated GO Term : protein-L-isoaspartate (D-aspartate) O-methyltransferase activity
KOG Annotation  Protein-L-isoaspartate(D-aspartate) O-methyltransferase
What's a KOG?
Evidence for Inhibition 
ePCR Status: Bottom50_percentile_ePCR (I:384765..386429)
Transcript overlap: 97 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 97
Relative score: 0.142 (Best raw score= for Gene )
Specificity index: 0.124
External "R119.5" Links  Wormbase NCBI
Simmer:R119.6:Screen_A Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 100 %
Larval  Gro
Adult  Ste brood size (1 out of 5)
Unc
Simmer:R119.6:Screen_A Experiment Details Ace View
Laboratory  NL
RNAi Reagent & Type  sjj_R119.6 (PCR_reagent)
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Date  2003-10-01
Gene Plot of R119.5 Browse using GBrowse
Gene Graph
Gene Plot of R119.6 Browse using GBrowse
Gene Graph