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RNAi Experiment: Simmer:T01G9.5:Screen_A Ace View of Simmer:T01G9.5:Screen_A
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  T01G9.5 (mei-1)
Description of "T01G9.5"  mei-1 encodes, by alternative splicing, two slightly different isoforms of an ortholog of the catalytic subunit of katanin (OMIM:606696) that is required for oocyte meiotic spindle formation, for stable association of the meiosis I spindle with the oocyte cortex, and for microtubule severing; MEI-1 exists in a physical complex with MEI-2 at the polar ends of meiotic spindle microtubules and meiotic chromatin; MEI-1 must be inactivated for meiosis to give way to mitosis, and ectopic expression of MEI-1/MEI-2 during mitosis is normally lethal; MEI-1 is bound by the BTB protein MEL-26 (both in vivo and in vitro), which physically links MEI-1 to CUL-3 (an E3 ubiquitin ligase); CUL-3, in turn, promotes MEI-1 proteolysis by the 26S proteasome during the transition from meiosis to mitosis; the MEI-1/MEI-2 complex requires tubules containing the germline-enriched beta tubulin TBB-2 in vivo, but does not require tubules made from TBB-2's (superficially) redundant paralog TBB-1.
Evidence for Inhibition 
ePCR Status: Unique_ePCR (I:8294952..8296117)
Transcript overlap: 1070 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 1007
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "T01G9.5" Links  Wormbase NCBI
Simmer:T01G9.5:Screen_A Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 100 %
Simmer:T01G9.5:Screen_A Experiment Details Ace View
Laboratory  NL
RNAi Reagent & Type  sjj_T01G9.5 (PCR_reagent)
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Date  2003-10-01
Gene Plot of T01G9.5 Browse using GBrowse
Gene Graph