RNAi Experiment: Simmer:T01G9.5:Screen_B |
Ace View of Simmer:T01G9.5:Screen_B
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Genes Inhibited |
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Gene Inhibited |
T01G9.5 (mei-1) |
Description of "T01G9.5" |
mei-1 encodes, by alternative splicing, two slightly different isoforms of an ortholog of the catalytic subunit of katanin (OMIM:606696) that is required for oocyte meiotic spindle formation, for stable association of the meiosis I spindle with the oocyte cortex, and for microtubule severing; MEI-1 exists in a physical complex with MEI-2 at the polar ends of meiotic spindle microtubules and meiotic chromatin; MEI-1 must be inactivated for meiosis to give way to mitosis, and ectopic expression of MEI-1/MEI-2 during mitosis is normally lethal; MEI-1 is bound by the BTB protein MEL-26 (both in vivo and in vitro), which physically links MEI-1 to CUL-3 (an E3 ubiquitin ligase); CUL-3, in turn, promotes MEI-1 proteolysis by the 26S proteasome during the transition from meiosis to mitosis; the MEI-1/MEI-2 complex requires tubules containing the germline-enriched beta tubulin TBB-2 in vivo, but does not require tubules made from TBB-2's (superficially) redundant paralog TBB-1. |
Evidence for Inhibition |
ePCR |
Status: Unique_ePCR (I:8294952..8296117)
Transcript overlap: 1070 nt |
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Sliding Window |
Raw score: 1007
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene) |
Wormbase (BLAST) |
RNAi primary |
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External "T01G9.5" Links |
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Simmer:T01G9.5:Screen_B Phenotypes |
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Embryonic |
Emb Penetrance 100 % |
Simmer:T01G9.5:Screen_B Experiment Details |
Ace View |
Laboratory |
NL |
RNAi Reagent & Type |
sjj_T01G9.5 (PCR_reagent) |
Strain |
NL4256 rrf-3(pk1426) |
Reference |
Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...." |
Date |
2003-10-01 |
Gene Plot of T01G9.5 |
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