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RNAi Experiment: Simmer:T09A5.10 Ace View of Simmer:T09A5.10
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  T09A5.10 (lin-5)
Description of "T09A5.10"  lin-5 encodes a novel protein with a centrally located alpha-helical coiled-coil domain and ten potential proline-directed kinase phosphorylation sites; LIN-5 activity is required for mitosis and cytokinesis at all stages of development, specifically for spindle positioning, chromosome alignment and segregation, and perhaps also for blocking exit from mitosis when chromosome segregation fails; LIN-5, which is expressed maternally, embryonically, and during larval stages, localizes to the cell periphery and, in microtubule-dependent manner, to the meiotic spindle and the asters and kinetochore microtubules of the mitotic spindle; in regulating spindle function, LIN-5 interacts with two G protein regulators, GPR-1 and GPR-2, which require LIN-5 activity for localization to the spindle and cell cortex; additionally, LIN-5 interacts in vitro with itself, the coiled-coil protein LFI-1, GEI-16, and several other novel proteins; genetic studies indicate that in regulating embryonic spindle function, lin-5 acts downsteam of par-2, par-3, mes-1, and src-1 and upstream of gpr-1, gpr-2, goa-1, and gpa-16.
Evidence for Inhibition 
ePCR Status: Unique_ePCR (II:7862092..7863230)
Transcript overlap: 932 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 848
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "T09A5.10" Links  Wormbase NCBI
Simmer:T09A5.10 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance 100 %
Simmer:T09A5.10 Experiment Details Ace View
Laboratory  NL
RNAi Reagent & Type  sjj_T09A5.10 (PCR_reagent)
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Date  2003-10-01
Gene Plot of T09A5.10 Browse using GBrowse
Gene Graph