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RNAi Experiment: Simmer:ZK770.3:Screen_B Ace View of Simmer:ZK770.3:Screen_B
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  ZK770.3 (inx-12)
Description of "ZK770.3"  inx-12 (innexin=invertebrate connexin analogue) encodes a protein of the innexin family; innexins are gap junction proteins similar in function, though dissimilar in sequence to the vertebrate connexins; innexins are the only known gap junction proteins in invertebrates; inx-12 and inx-13 appear to be a tandom duplication; RNA interference of inx-12 results in an arrest at the L1 larval stage with fluid filled dead rods; this phenotype and sequence homology to Drosophila innexins suggest that INX-12 may be required to form gap junctions between the excretory canal and hypodermis, thus playing an essential role in osmoregulation; an INX-12::GFP fusion protein is expressed in the excretory canal.
Evidence for Inhibition 
ePCR Status: Unique_ePCR (I:3755369..3757487)
Transcript overlap: 905 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 822
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "ZK770.3" Links  Wormbase NCBI
Simmer:ZK770.3:Screen_B Phenotypes Help with Phenotypes
Larval  Gro could not be confirmed by re-testing
Adult  Pvl could not be confirmed by re-testing
Rup could not be confirmed by re-testing
Stp could not be confirmed by re-testing
Unc could not be confirmed by re-testing
Simmer:ZK770.3:Screen_B Experiment Details Ace View
Laboratory  NL
RNAi Reagent & Type  sjj_ZK770.3 (PCR_reagent)
Strain  NL4256 rrf-3(pk1426)
Reference  Simmer F et al. (2003) PLoS Biology "Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals ...."
Date  2003-10-01
Gene Plot of ZK770.3 Browse using GBrowse
Gene Graph