RNAi Experiment: TH:300c6 |
Ace View of TH:300c6
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Genes Inhibited |
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Gene Inhibited |
C36E8.5 (tbb-2) |
Description of "C36E8.5" |
tbb-2 encodes a homolog of mammalian beta-tubulin (TUBB) that is expressed at high levels in the germline; TBB-2 is redundant for embryonic viability, due to its paralog TBB-1; however, unlike its paralog TBB-1 it is preferred for normal microtubule severing by the katanin complex MEI-1/MEI-2; TBB-2 is required together with TBB-1, TBA-1, and tba-2 to make spindle structures in embryos and TBB-2 also affects the stability of the PO spindle; first divisions are normal in null mutants, but putative gain-of-function mutations affect centrosome rotations in the P0, P1, and EMS blastomeres of the early embryo, and affect pronuclear migration and meiosis. |
KOG Annotation  |
Beta tubulin
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Evidence for Inhibition |
ePCR |
Status: Unique_ePCR (III:4015893..4017836)
Transcript overlap: 1511 nt |
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Sliding Window |
Raw score: 1385
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.951 |
Wormbase (BLAST) |
RNAi primary |
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External "C36E8.5" Links |
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Gene Inhibited |
C54C6.2 (ben-1) |
Description of "C54C6.2" |
A beta-tubulin that confers benzimidazole sensitivity. |
KOG Annotation  |
Beta tubulin
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Evidence for Inhibition |
Sliding Window |
Raw score: 72
Relative score: 0.052 (Best raw score= for Gene )
Specificity index: 0.049 |
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External "C54C6.2" Links |
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TH:300c6 Phenotypes |
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Embryonic |
Emb Penetrance None reported |
TH:300c6 Experiment Details |
Ace View |
Laboratory |
TH |
RNAi Reagent & Type |
TH:300c6 (PCR_reagent) |
Remark |
Phenotype comment -- Confirmed with independent dsRNA (C36E8.5-RNA2; similar phenotype). Phenotype could potentially be due to cross-RNAi inhibition of other tubulin genes (see BLASTN results). We note, however, that RNAi directed specifically against one other tubulin gene on chromosome III (C54C6.2; ben-1) failed to give rise to a phenotype, suggesting that the phenotype noted here may truly reflect a requirement for C36E8.5 function. |
Reference |
Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...." |
Date |
2000-11-16 |
Gene Plot of C54C6.2 |
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Gene Plot of C36E8.5 |
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