RNAi Experiment: TH:302e10 |
Ace View of TH:302e10
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Genes Inhibited |
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Gene Inhibited |
C14B9.4 (plk-1) |
Description of "C14B9.4" |
plk-1 encodes a serine/threonine polo-like kinase homologous to Drosophila polo and Saccharomyces cerevisiae CDC5; PLK-1 is required for meiotic nuclear envelope breakdown, polar body formation and extrusion, and proper chromosome segregation during meiosis, and additionally, may play a role in germline development; PLK-1 is proposed to phosphorylate REC-8, a meiotic-specific cohesin, thus promoting REC-8 degradation and subsequent separation of sister chromatids; PLK-1 is localized diffusely in the cytoplasm of mature oocytes, but during meiosis I becomes associated with pre-metaphase chromatin and then at anaphase with chromatin and the area between dividing chromatin; in embryos, PLK-1 localizes to centrosomes, metaphase chromatin, and during telophase, to the area between dividing chromatin. |
Evidence for Inhibition |
ePCR |
Status: Unique_ePCR (III:8100579..8102310)
Transcript overlap: 849 nt |
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Sliding Window |
Raw score: 744
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene) |
Wormbase (BLAST) |
RNAi primary |
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External "C14B9.4" Links |
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TH:302e10 Phenotypes |
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Embryonic |
Emb Penetrance None reported |
TH:302e10 Experiment Details |
Ace View |
Laboratory |
TH |
RNAi Reagent & Type |
TH:302e10 (PCR_reagent) |
Remark |
Phenotype comment -- Confirmed with independent dsRNA against K06H7.1 (K06H7.1-RNA1); see also Chase et al. (2000). A shorter dsRNA against K06H7.1, covering exons 3 and 4 (K06H7.1-RNA2), did not result in a phenotype, suggesting inaccurate gene prediction. |
Reference |
Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...." |
Date |
2000-11-16 |
Gene Plot of C14B9.4 |
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