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RNAi Experiment: TH:302f10 Ace View of TH:302f10
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C14B9.4 (plk-1)
Description of "C14B9.4"  plk-1 encodes a serine/threonine polo-like kinase homologous to Drosophila polo and Saccharomyces cerevisiae CDC5; PLK-1 is required for meiotic nuclear envelope breakdown, polar body formation and extrusion, and proper chromosome segregation during meiosis, and additionally, may play a role in germline development; PLK-1 is proposed to phosphorylate REC-8, a meiotic-specific cohesin, thus promoting REC-8 degradation and subsequent separation of sister chromatids; PLK-1 is localized diffusely in the cytoplasm of mature oocytes, but during meiosis I becomes associated with pre-metaphase chromatin and then at anaphase with chromatin and the area between dividing chromatin; in embryos, PLK-1 localizes to centrosomes, metaphase chromatin, and during telophase, to the area between dividing chromatin.
Evidence for Inhibition 
ePCR Status: Unique_ePCR (III:8102448..8104613)
Transcript overlap: 1407 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 1323
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "C14B9.4" Links  Wormbase NCBI
TH:302f10 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
TH:302f10 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  TH:302f10 (PCR_reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA against K06H7.1 (K06H7.1-RNA1); see also Chase et al. (2000). A shorter dsRNA against K06H7.1, covering exons 3 and 4 (K06H7.1-RNA2), did not result in a phenotype, suggesting inaccurate gene prediction.
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Date  2000-11-16
Gene Plot of C14B9.4 Browse using GBrowse
Gene Graph