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RNAi Experiment: TH:305A12 Ace View of TH:305A12
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F10E9.8 (sas-4)
Description of "F10E9.8"  sas-4 encodes a predicted coiled-coil protein and centriole component recruited to the centrosome once per cell cycle at the time of organelle duplication and is required for centriole duplication and spindle assembly, levels dictate centrosome size, and also affects germ cell proliferation and locomotion; colocalizes with gamma-tubulin to centrosomes both in sperm and in the syncitial part of the gonad.
Evidence for Inhibition 
ePCR Status: Best_ePCR (III:8316670..8318783)
Transcript overlap: 1364 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 1217
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.908
Wormbase (BLAST) RNAi primary
External "F10E9.8" Links  Wormbase NCBI
Gene Inhibited  F10E9.11
KOG Annotation  Uncharacterized conserved protein
What's a KOG?
Evidence for Inhibition 
ePCR Status: Bottom50_percentile_ePCR (III:8316670..8318783)
Transcript overlap: 124 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 124
Relative score: 0.102 (Best raw score= for Gene )
Specificity index: 0.092
Wormbase (BLAST) RNAi primary
External "F10E9.11" Links  Wormbase NCBI
TH:305A12 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
TH:305A12 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  TH:305A12 (PCR_reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (F10E9.8-RNA2); not confirmed with third dsRNA (F10E9.8-RNA3).
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Date  2000-11-16
Gene Plot of F10E9.11 Browse using GBrowse
Gene Graph
Gene Plot of F10E9.8 Browse using GBrowse
Gene Graph