RNAi Experiment: TH:307C1 |
Ace View of TH:307C1
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Genes Inhibited |
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Gene Inhibited |
C02F5.1 (knl-1) |
Description of "C02F5.1" |
knl-1 encodes a novel acidic protein with a coiled-coil region at its C-terminus; KNL-1 is an essential kinetochore component that is required for proper spindle elongation and chromosome separation, and in the kinetochore assembly pathway, plays a key role in linking the initiation of kinetochore formation with the construction of a functional microtubule-binding interface; in the assembly pathway, KNL-1 functions downstream of the DNA-proximal kinetochore components CeCENP-A/HCP-3 and CeCENP-B/HCP-4 and upstream of the outer kinetochore components HIM-10/Nuf2p, NDC-80/HEC1, CeBUB-1, HCP-1, and CeCLASP2/CLS-2; in expression studies in the one-cell embryo, KNL-1 localizes to kinetochores throughout mitosis. |
Evidence for Inhibition |
ePCR |
Status: Unique_ePCR (III:8253234..8254022)
Transcript overlap: 625 nt |
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Sliding Window |
Raw score: 604
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene) |
Wormbase (BLAST) |
RNAi primary |
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External "C02F5.1" Links |
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TH:307C1 Phenotypes |
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Embryonic |
Emb Penetrance None reported |
TH:307C1 Experiment Details |
Ace View |
Laboratory |
TH |
RNAi Reagent & Type |
TH:307C1 (PCR_reagent) |
Remark |
Phenotype comment -- Confirmed with independent dsRNA (C02F5.1-RNA2; similar DIC phenotype, progeny test not conducted). |
Reference |
Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...." |
Date |
2000-11-16 |
Gene Plot of C02F5.1 |
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