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RNAi Experiment: TH:314G1 Ace View of TH:314G1
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  T04A8.14 (emb-5)
Description of "T04A8.14"  emb-5 encodes the C. elegans ortholog of the Spt6 family of RNA polymerase II transcription elongation factors; first identified in screens for temperature-sensitive embryonic lethal mutations, emb-5 is required for the correct timing of the second E (endoderm)-cell division in the early embryo and thus, for normal embryonic gastrulation; in addition, emb-5 activity is required postembryonically for proper gonad development; in yeast two-hybrid studies, EMB-5 interacts with the intracellular domains of LIN-12 and GLP-1, suggesting that EMB-5 functions as a positive downstream effector in Notch-like signaling pathways during C. elegans development; emb-5 mRNA is most abundant during embryonic stages, with lower levels apparent during the L1-L3 larval stages, and even lower levels observed during L4.
KOG Annotation  Transcription elongation factor SPT6
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (III:4717904..4719525)
Transcript overlap: 1478 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 1394
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "T04A8.14" Links  Wormbase NCBI
TH:314G1 Phenotypes Help with Phenotypes
Phenotype  WTDIC phenotype -- Embryonic development normal
TH:314G1 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  TH:314G1 (PCR_reagent)
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Delivery Method  Injection
Date  2000-11-16
Gene Plot of T04A8.14 Browse using GBrowse
Gene Graph