RNAi Experiment: TH:320a9 |
Ace View of TH:320a9
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Genes Inhibited |
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Gene Inhibited |
K08E7.3 (let-99) |
Description of "K08E7.3" |
let-99 encodes a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); LET-99 has no obvious homologs in non-nematodes, but has a truncated paralog (LRG-1) in C. elegans; LET-99 is required for the mitotic spindle to be properly oriented with respect to the axis of cellular polarity, both during anterior migration and rotation of the nuclear-centrosome complex and during anaphase; let-99 mutants exhibit hyperactive nuclear movement and abnormal anaphase spindle pole behavior; LET-99 is mislocalized in par-2 and par-3 mutants; like other proteins containing DEP domains, LET-99 may act as part of a G protein signalling pathway (e.g., the one controlling spindle position). |
Evidence for Inhibition |
Sliding Window |
Raw score: 135
Relative score: 0.34 (Best raw score= for Gene )
Specificity index: 0.254 |
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External "K08E7.3" Links |
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Gene Inhibited |
F55H2.4 (lrg-1) |
Description of "F55H2.4" |
lrg-1 encodes a truncated paralog of let-99, a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); lrg-1(RNAi) animals have no obvious phenotype; since the nucleotide similarity of lrg-1 to let-99 extends beyond the lrg-1 CDS, and since lrg-1 has no obvious function in RNAi assays, this gene may be decaying towards pseudogene status. |
Evidence for Inhibition |
Sliding Window |
Raw score: 397
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.746 |
Wormbase (BLAST) |
RNAi primary |
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External "F55H2.4" Links |
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TH:320a9 Phenotypes |
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Embryonic |
Emb Penetrance None reported |
TH:320a9 Experiment Details |
Ace View |
Laboratory |
TH |
Author |
Gonczy P; Echeverri C; Oegema K; Coulson AR; Jones SJ; Copley RR et al. |
RNAi Reagent & Type |
TH:320a9 (PCR_reagent) |
Remark |
Phenotype comment -- Confirmed with dsRNA against F55H2.4 (F55H2.4-RNA1; similar phenotype). Phenotype may be due to cross-RNAi inhibition of let-99 (K08E7.3): see BLASTN data and Rose and Kemphues (1997). |
Date |
2000-11-16 |
Gene Plot of K08E7.3 |
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Gene Plot of F55H2.4 |
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