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RNAi Experiment: TH:320b9 Ace View of TH:320b9
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  K08E7.3 (let-99)
Description of "K08E7.3"  let-99 encodes a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); LET-99 has no obvious homologs in non-nematodes, but has a truncated paralog (LRG-1) in C. elegans; LET-99 is required for the mitotic spindle to be properly oriented with respect to the axis of cellular polarity, both during anterior migration and rotation of the nuclear-centrosome complex and during anaphase; let-99 mutants exhibit hyperactive nuclear movement and abnormal anaphase spindle pole behavior; LET-99 is mislocalized in par-2 and par-3 mutants; like other proteins containing DEP domains, LET-99 may act as part of a G protein signalling pathway (e.g., the one controlling spindle position).
Evidence for Inhibition 
Sliding Window Raw score: 171
Relative score: 0.151 (Best raw score= for Gene )
Specificity index: 0.131
External "K08E7.3" Links  Wormbase NCBI
Gene Inhibited  F55H2.4 (lrg-1)
Description of "F55H2.4"  lrg-1 encodes a truncated paralog of let-99, a protein with a DEP domain (Domain found in Dishevelled, Egl-10, and Pleckstrin); lrg-1(RNAi) animals have no obvious phenotype; since the nucleotide similarity of lrg-1 to let-99 extends beyond the lrg-1 CDS, and since lrg-1 has no obvious function in RNAi assays, this gene may be decaying towards pseudogene status.
Evidence for Inhibition 
Sliding Window Raw score: 1135
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.869
Wormbase (BLAST) RNAi primary
External "F55H2.4" Links  Wormbase NCBI
TH:320b9 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
TH:320b9 Experiment Details Ace View
Laboratory  TH
Author  Gonczy P; Echeverri C; Oegema K; Coulson AR; Jones SJ; Copley RR et al.
RNAi Reagent & Type  TH:320b9 (PCR_reagent)
Remark  Phenotype comment -- Confirmed with dsRNA against F55H2.4 (F55H2.4-RNA1; similar phenotype). Phenotype may be due to cross-RNAi inhibition of let-99 (K08E7.3): see BLASTN data and Rose and Kemphues (1997).
Date  2000-11-16
Gene Plot of K08E7.3 Browse using GBrowse
Gene Graph
Gene Plot of F55H2.4 Browse using GBrowse
Gene Graph