Link to F56A8.3a.1Link to F56A8.3a.2Link to F56A8.3b.1Link to F56A8.3b.2RNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentgeneMap Link to B0464.5b.1Link to B0464.5c.1Link to B0464.5c.2Link to B0464.5b.2Link to B0464.5aRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAiRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentRNAi ReagentgeneMap
Go to RNAiDB Home Adult Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie Wild-type C. elegans Movie
 
RNAi Experiment: TH:320d1 Ace View of TH:320d1
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F56A8.3
KOG Annotation  Leucine-rich repeat protein
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (III:13256129..13257196)
Transcript overlap: 602 nt		 Explain types of evidence for RNAi inhibition
External "F56A8.3" Links  Wormbase NCBI
Gene Inhibited  B0464.5 (spk-1)
Description of "B0464.5"  spk-1 encodes a serine/threonine kinase orthologous to the highly conserved serine/arginine-rich protein kinases (SRPKs) that phosphorylate SR proteins, essential components of the pre-mRNA splicing machinery; SPK-1, required for embryogenesis and germline development, can directly bind and phosphorylate RSP-3 (CeSF2/ASF), an SR splicing factor homolog; SPK-1 is expressed predominantly in the adult germline; SPK-1 is homologous to human RSK2, which when mutated leads to Coffin-Lowry syndrome (OMIM:303600).
KOG Annotation  Serine/threonine protein kinase
What's a KOG?
Evidence for Inhibition 
Sliding Window Raw score: 1478
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "B0464.5" Links  Wormbase NCBI
TH:320d1 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
TH:320d1 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  TH:320d1 (PCR_reagent)
Remark  Phenotype comment -- Confirmed in part with independent dsRNA (B0464.5-RNA2; weaker DIC phenotype: cleavage furrow ingresses with a delay; excess cortical contractions, little rocking during anaphase, failure in P1 rotation; only partial embryonic lethal).
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Date  2000-11-16
Gene Plot of F56A8.3 Browse using GBrowse
Gene Graph
Gene Plot of B0464.5 Browse using GBrowse
Gene Graph