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RNAi Experiment: TH:322D5_2 Ace View of TH:322D5_2
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  K08E3.8 (mdt-29)
Description of "K08E3.8"  mdt-29 encodes a putative mediator subunit with a prion-like (asparagine- or glutamine-rich) domain; note that K08E3.8 is allegedly an ortholog of Drosophila INTERSEX/IX, but the actual C. elegans IX ortholog appears to be PQN-15); in yeast two-hybrid screens, MDT-29 interacts with SEL-7 and SEL-8; MDT-29 protein also self-associates; RNAi of K08E3.8 weakly enhances the two-anchor-cell phenotype of lin-12(ar170), roughly as much as sel-7 RNAi; since SEL-7 is a novel nuclear protein, MDT-29/SEL-7/SEL-8 is likely to form a nuclear complex formed in response to LIN-12 activation.
Evidence for Inhibition 
ePCR Status: Best_ePCR (III:13776330..13777229)
Transcript overlap: 335 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 293
Relative score: 0.936 (Best raw score= for Gene )
Specificity index: 0.41
Wormbase (BLAST) RNAi primary
External "K08E3.8" Links  Wormbase NCBI
Gene Inhibited  C04H5.9
Evidence for Inhibition 
Sliding Window Raw score: 109
Relative score: 0.348 (Best raw score= for Gene )
Specificity index: 0.152
External "C04H5.9" Links  Wormbase NCBI
Gene Inhibited  3R5.2
Evidence for Inhibition 
Sliding Window Raw score: 313
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.438
External "3R5.2" Links  Wormbase NCBI
TH:322D5_2 Phenotypes Help with Phenotypes
Phenotype  WTDIC phenotype -- Embryonic development normal
TH:322D5_2 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  TH:322D5_2 (PCR_reagent)
Remark  This RNAi has multiple targets in the genome, see also RNAi: TH:322D5_1
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Delivery Method  Injection
Date  2000-11-16
Gene Plot of K08E3.8 Browse using GBrowse
Gene Graph
Gene Plot of C04H5.9 Browse using GBrowse
Gene Graph
Gene Plot of 3R5.2 Browse using GBrowse
Gene Graph