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RNAi Experiment: TH:322d11 Ace View of TH:322d11
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F54C8.2
Description of "F54C8.2"  Associated GO Term : DNA binding
KOG Annotation  Histones H3 and H4
What's a KOG?
Evidence for Inhibition 
ePCR Status: Best_ePCR (III:9433794..9435113)
Transcript overlap: 786 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 702
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.808
Wormbase (BLAST) RNAi primary
External "F54C8.2" Links  Wormbase NCBI
Gene Inhibited  F54C8.3 (emb-30)
Description of "F54C8.3"  emb-30 encodes an anaphase-promoting complex/cyclosome (APC/C) component orthologous to mammalian APC-4 and Schizosaccharomyces pombe Lid1; EMB-30 is required for the metaphase-to-anaphase transition during meiosis and mitosis, for establishing anterior-posterior polarity in the early embryo, and for proper localization of germline granules and the maternally provided PAR-2 and PAR-3 proteins.
Evidence for Inhibition 
ePCR Status: Bottom50_percentile_ePCR (III:9433794..9435113)
Transcript overlap: 135 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 93
Relative score: 0.132 (Best raw score= for Gene )
Specificity index: 0.107
External "F54C8.3" Links  Wormbase NCBI
Gene Inhibited  F58A4.3 (hcp-3)
Description of "F58A4.3"  The hcp-3 gene encodes a centromere protein (CENP)-A homolog required for kinetochore function; inactivation of hcp-3 in one-cell embryos by RNAi causes a complete loss of kinetochores, with total failure of chromosomes to segregate properly during mitosis, to recruit components to the kinetochore other than HCP-3, or to assemble a stable mitotic spindle; in addition, HCP-4 fails to localize properly to the kinetochore in hcp-3(RNAi) embryos.
KOG Annotation  Histones H3 and H4
What's a KOG?
Evidence for Inhibition 
Sliding Window Raw score: 74
Relative score: 0.105 (Best raw score= for Gene )
Specificity index: 0.085
External "F58A4.3" Links  Wormbase NCBI
TH:322d11 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
TH:322d11 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  TH:322d11 (PCR_reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (K.O., unpublished observations; similar phenotype). Phenotype could potentially be due to cross-RNAi inhibition of other CENP-A like gene (F58A4.3) (see BLASTN results). We note, however, that the phenotypes associated with these two genes are somewhat different, suggesting that the phenotype noted here may truly reflect a requirement for F54C8.2 function.
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Date  2000-11-16
Gene Plot of F54C8.3 Browse using GBrowse
Gene Graph
Gene Plot of F58A4.3 Browse using GBrowse
Gene Graph
Gene Plot of F54C8.2 Browse using GBrowse
Gene Graph