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RNAi Experiment: TH:340f4 Ace View of TH:340f4
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C38C10.4 (gpr-2)
Description of "C38C10.4"  gpr-2 encodes a protein containing a GPR (G Protein Regulator)/GoLoco motif characteristic of guanine nucleotide exchange factors specific for G-alpha GTPases; GPR-2 appears to function redundantly during early embryogenesis and germ-line development to regulate chromosome and spindle movements during cell division; GPR-2 likely acts as a positive regulator of G protein signaling and specifically, may regulate GOA-1 signaling in the embryo; GPR-2 forms a protein complex with the nearly identical GPR-1 and with LIN-5, a coiled-coil protein that is required for proper localization of GPR-2 to the cell cortex and spindle asters of the early embryo; in addition, proper GPR-2/GPR-1/LIN-5 localization between the P2 and EMS blastomeres at the four-cell stage, which may contribute to spindle positioning in EMS, requires the MES-1/SRC-1 tyrosine kinase signaling pathway; GPR-2 is believed to act downstream of, or in parallel to, PAR-3, a PDZ domain-containing protein, in mitotic spindle positioning in the early embryo.
Evidence for Inhibition 
ePCR Status: Best_ePCR (III:9391310..9392181)
Transcript overlap: 820 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 778
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.608
Wormbase (BLAST) RNAi primary
External "C38C10.4" Links  Wormbase NCBI
Gene Inhibited  F22B7.13 (gpr-1)
Description of "F22B7.13"  Associated GO Term : GTPase activator activity
Evidence for Inhibition 
ePCR Status: Best_ePCR (III:8629714..8630585)
Transcript overlap: 820 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 502
Relative score: 0.645 (Best raw score= for Gene )
Specificity index: 0.392
External "F22B7.13" Links  Wormbase NCBI
TH:340f4 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
TH:340f4 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  TH:340f4 (PCR_reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (C38C10.4-RNA1; similar phenotype). Phenotype may be due to cross-RNAi inhibition of F22B7.13 (see BLASTN data).
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Date  2000-11-16
Gene Plot of C38C10.4 Browse using GBrowse
Gene Graph
Gene Plot of F22B7.13 Browse using GBrowse
Gene Graph