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RNAi Experiment: TH:341d11 Ace View of TH:341d11
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C36E8.5 (tbb-2)
Description of "C36E8.5"  tbb-2 encodes a homolog of mammalian beta-tubulin (TUBB) that is expressed at high levels in the germline; TBB-2 is redundant for embryonic viability, due to its paralog TBB-1; however, unlike its paralog TBB-1 it is preferred for normal microtubule severing by the katanin complex MEI-1/MEI-2; TBB-2 is required together with TBB-1, TBA-1, and tba-2 to make spindle structures in embryos and TBB-2 also affects the stability of the PO spindle; first divisions are normal in null mutants, but putative gain-of-function mutations affect centrosome rotations in the P0, P1, and EMS blastomeres of the early embryo, and affect pronuclear migration and meiosis.
KOG Annotation  Beta tubulin
What's a KOG?
Evidence for Inhibition 
ePCR Status: Unique_ePCR (III:4016333..4016531)
Transcript overlap: 199 nt		 Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 136
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "C36E8.5" Links  Wormbase NCBI
TH:341d11 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
TH:341d11 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  TH:341d11 (PCR_reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (C36E8.5-RNA2; similar phenotype). Phenotype could potentially be due to cross-RNAi inhibition of other tubulin genes (see BLASTN results). We note, however, that RNAi directed specifically against one other tubulin gene on chromosome III (C54C6.2; ben-1) failed to give rise to a phenotype, suggesting that the phenotype noted here may truly reflect a requirement for C36E8.5 function.
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Date  2000-11-16
Gene Plot of C36E8.5 Browse using GBrowse
Gene Graph