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RNAi Experiment: TH:343A4 Ace View of TH:343A4
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C02F5.1 (knl-1)
Description of "C02F5.1"  knl-1 encodes a novel acidic protein with a coiled-coil region at its C-terminus; KNL-1 is an essential kinetochore component that is required for proper spindle elongation and chromosome separation, and in the kinetochore assembly pathway, plays a key role in linking the initiation of kinetochore formation with the construction of a functional microtubule-binding interface; in the assembly pathway, KNL-1 functions downstream of the DNA-proximal kinetochore components CeCENP-A/HCP-3 and CeCENP-B/HCP-4 and upstream of the outer kinetochore components HIM-10/Nuf2p, NDC-80/HEC1, CeBUB-1, HCP-1, and CeCLASP2/CLS-2; in expression studies in the one-cell embryo, KNL-1 localizes to kinetochores throughout mitosis.
Evidence for Inhibition 
Sliding Window Raw score: 1288
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "C02F5.1" Links  Wormbase NCBI
TH:343A4 Phenotypes Help with Phenotypes
Embryonic  Emb Penetrance None reported
TH:343A4 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  TH:343A4 (PCR_reagent)
Remark  Phenotype comment -- Confirmed with independent dsRNA (C02F5.1-RNA2; similar DIC phenotype, progeny test not conducted).
Reference  Gonczy P et al. (2000) Nature "Functional genomic analysis of cell division in C. elegans using RNAi of genes on ...."
Date  2000-11-16
Gene Plot of C02F5.1 Browse using GBrowse
Gene Graph