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RNAi Experiment: WBRNAi00038543 Ace View of WBRNAi00038543
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  F32H2.3 (spd-2)
Description of "F32H2.3"  spd-2 encodes a protein with three predicted coiled-coil domains; SPD-2 activity is essential for centrosome maturation and duplication, and hence for proper mitotic spindle assembly; in regulating the early steps of centrosome maturation, SPD-2 acts together with the coiled-coil protein SPD-5, the Aurora-A kinase AIR-1, and the cytoplasmic dynein DHC-1; in regulating centrosome duplication, SPD-2 likely acts together with the ZYG-1 kinase; in both processes, SPD-2 may function to recruit centrosomal components such as ZYG-9 and microtubule-nucleating gamma-tubulin complexes to the developing centrosome; within centrosomes, SPD-2 localizes to both centrioles and the pericentriolar material; efficient centrosomal localization depends upon AIR-1 and DHC-1, while localization specifically to the pericentriolar material depends upon SPD-5.
Evidence for Inhibition 
ePCR Status: Unique_ePCR (I:8969088..8970166)
Transcript overlap: 900 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 816
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "F32H2.3" Links  Wormbase NCBI
WBRNAi00038543 Phenotypes Help with Phenotypes
Phenotype  Unclassified
WBRNAi00038543 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  WBPaper00024346:F32H2_1.1 (PCR_reagent)
Remark  In spd-2(RNAi) embryos, GFP-PAR-2 was found only in the cytoplasm and ruffling occurred throughout the entire embryo cortex indicating failure of polarity establishment.
Reference  Cowan CR et al. (2004) Nature "Centrosomes direct cell polarity independently of microtubule assembly in C. ...."
Delivery Method  Injection
Date  2004-9-02
Gene Plot of F32H2.3 Browse using GBrowse
Gene Graph