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RNAi Experiment: WBRNAi00038545 Ace View of WBRNAi00038545
Genes Inhibited How is RNAi inhibition determined?
Gene Inhibited  C36E8.5 (tbb-2)
Description of "C36E8.5"  tbb-2 encodes a homolog of mammalian beta-tubulin (TUBB) that is expressed at high levels in the germline; TBB-2 is redundant for embryonic viability, due to its paralog TBB-1; however, unlike its paralog TBB-1 it is preferred for normal microtubule severing by the katanin complex MEI-1/MEI-2; TBB-2 is required together with TBB-1, TBA-1, and tba-2 to make spindle structures in embryos and TBB-2 also affects the stability of the PO spindle; first divisions are normal in null mutants, but putative gain-of-function mutations affect centrosome rotations in the P0, P1, and EMS blastomeres of the early embryo, and affect pronuclear migration and meiosis.
KOG Annotation  Beta tubulin
What's a KOG?
Evidence for Inhibition 
ePCR Status: Top85_percentile_ePCR (III:4016326..4017329)
Transcript overlap: 927 nt
Explain types of evidence for RNAi inhibition
Sliding Window Raw score: 843
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)
Wormbase (BLAST) RNAi primary
External "C36E8.5" Links  Wormbase NCBI
Gene Inhibited  F44F4.11 (tba-4)
Description of "F44F4.11"  tba-4 encodes a member of the alpha tubulin family that also includes tba-1, tba-2, and tba-3, and affects embryonic viability.
KOG Annotation  Alpha tubulin
What's a KOG?
Evidence for Inhibition 
ePCR Transcript overlap: 1004 nt Explain types of evidence for RNAi inhibition
Wormbase (BLAST) RNAi primary
External "F44F4.11" Links  Wormbase NCBI
WBRNAi00038545 Phenotypes Help with Phenotypes
Phenotype  Unclassified
WBRNAi00038545 Experiment Details Ace View
Laboratory  TH
RNAi Reagent & Type  WBPaper00024346:C36E8_2.1 (PCR_reagent)
Remark  Depletion of alpha/beta-tubulin by RNAi did not affect polarity.
Reference  Cowan CR et al. (2004) Nature "Centrosomes direct cell polarity independently of microtubule assembly in C. ...."
Delivery Method  Injection
Date  2004-9-02
Gene Plot of C36E8.5 Browse using GBrowse
Gene Graph
Gene Plot of F44F4.11 Browse using GBrowse
Gene Graph