RNAi Card Page

RNAi Experiment: [cgc3752]:cdk-4

Ace View of [cgc3752]:cdk-4

Genes Inhibited

Gene Inhibited

F19B2.4 (srz-36)

Evidence for Inhibition

Sliding Window

Raw score: 92
Relative score: 0.093 (Best raw score= for Gene )
Specificity index: 0.066

External "F19B2.4" Links

Gene Inhibited

F18H3.5 (cdk-4)

Description of "F18H3.5"

cdk-4 encodes two isoforms of a cyclin-dependent serine/threonine protein kinase orthologous to human CDK4 and CDK6 (OMIM:123829 and OMIM:603368, mutated in cutanous malignant melanoma) which complex with D-type cyclins to regulate progression through the G1 phase of the cell cycle; CDK-4 activity is essential for G1 progression in postembryonic blast cells and as a result, cdk-4 mutant animals generally arrest during larval stages; the lethality generated by cdk mutations, also seen in animals doubly mutant for cdk-4 and cyd-1, a C. elegans D-type cyclin, can be suppressed by mutations in lin-35/Rb suggesting that, as in other organisms, LIN-35/Rb may be a major target of CDK-4/CYD-1 kinase activity; CDK-4 expression is first detected in neuronal and hypodermal lineages during mid-to-late embryogenesis, with postembryonic expression detected in hypodermal seam cells, cells of the P lineage which will give rise to ventral cord neurons, and cells of the somatic gonad, the vulva, and the intestine.

KOG Annotation

Protein kinase PCTAIRE and related kinases

Evidence for Inhibition

Sliding Window	Raw score: 990 Relative score: 1 (This gene has the best score for this RNAi reagent) Specificity index: 0.706
Wormbase (BLAST)	RNAi primary

External "F18H3.5" Links

Gene Inhibited

F41H8.3 (srx-30)

Evidence for Inhibition

Sliding Window

Raw score: 320
Relative score: 0.323 (Best raw score= for Gene )
Specificity index: 0.228

External "F41H8.3" Links

[cgc3752]:cdk-4 Phenotypes

Larval

Lvl Low penetrance L2 arrest

Adult

Pvl
Unc

[cgc3752]:cdk-4 Experiment Details

Ace View

Laboratory

RNAi Reagent & Type

yk76f3 (cDNA_reagent)

Remark

defects are due to problems in cell proliferation: post-embryonic somatic blast cells sometimes fail to divide, including P blast cells, mesoblast M, somatic gonad precursor Z1 and Z4, and the intestinal nuclei.

Reference

Park M et al. (1999) Development "Regulation of postembryonic G1 cell cycle progression in Caenorhabditis ...."

Delivery Method

Injection

Date

1999-10-06

Gene Plot of F19B2.4

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Gene Plot of F18H3.5

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Gene Plot of F41H8.3

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