| RNAi Experiment: [cgc5320]:gip-1 |
Ace View of [cgc5320]:gip-1
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| Genes Inhibited |
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| Gene Inhibited |
H04J21.3 (gip-1) |
| Description of "H04J21.3" |
gip-1 encodes the C. elegans homolog of Saccharomyces cerevisiae Spc98p, a gamma-tubulin-binding protein that with Spc97p forms part of a gamma-tubulin ring complex that can nucleate microtubule formation in vitro and in vivo; in C. elegans, GIP-1 is essential for embryogenesis and required for formation of centrosomal asters during interphase and formation of the mitotic spindle, but not for formation of mitotic asters; GIP-1 colocalizes with TBG-1/gamma-tubulin to centrosomes during interphase and mitosis; GIP-1 and TBG-1 are required reciprocally for centrosomal localization. |
| Evidence for Inhibition |
| Sliding Window |
Raw score: 2671
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene) |
| Wormbase (BLAST) |
RNAi primary |
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| External "H04J21.3" Links |
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| [cgc5320]:gip-1 Phenotypes |
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| Embryonic |
Emb Penetrance None reported |
| [cgc5320]:gip-1 Experiment Details |
Ace View |
| Laboratory |
TH |
| RNAi Reagent & Type |
yk558c5 (cDNA_reagent) |
| Strain |
WT |
| Remark |
During interphase, cytoplasmic MTs were not affected, but assembly of centrosomal asters failed. Centrosomal asters formed as embryos entered mitosis, but spindle assembly never occurred. gamma-tubulin failed to locolize to centrosomes in gip-1(RNAi) embryos. |
| Reference |
Hannak E et al. (2002) Journal of Cell Biology "The kinetically dominant assembly pathway for centrosomal asters in ...." |
| Delivery Method |
Injection |
| Date |
2002-3-26 |
| Gene Plot of H04J21.3 |
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