RNAi Experiment: [cgc5655]:daf-2:K10B3.7 |
Ace View of [cgc5655]:daf-2:K10B3.7
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Genes Inhibited |
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Gene Inhibited |
K10B3.7 (gpd-3) |
Description of "K10B3.7" |
gpd-3 encodes a predicted glyceraldehyde 3-phosphate dehydrogenase that affects embryonic viability; GPD-3 interacts with LIN-2 in two-hybrid assays. |
KOG Annotation  |
Glyceraldehyde 3-phosphate dehydrogenase
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Evidence for Inhibition |
ePCR |
Status: Unique_ePCR (X:3112282..3113406)
Transcript overlap: 996 nt |
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Sliding Window |
Raw score: 893
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 0.464 |
Wormbase (BLAST) |
RNAi primary |
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External "K10B3.7" Links |
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Gene Inhibited |
K10B3.9 (mai-1) |
Description of "K10B3.9" |
mai-1 encodes a protein with similarity to human mitochondrial ATPase inhibitor; exists as the upstream gene in an operon with gpd-2 and gpd-3. |
Evidence for Inhibition |
Sliding Window |
Raw score: 527
Relative score: 0.59 (Best raw score= for Gene )
Specificity index: 0.274 |
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External "K10B3.9" Links |
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Gene Inhibited |
K10B3.8 (gpd-2) |
Description of "K10B3.8" |
gpd-2 encodes one of four C. elegans glyceraldehyde-3-phosphate dehydrogenases (GAPDHs); by homology, GPD-2 is predicted to reversibly catalyze the oxidation and phosphorylation of glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate during glycolysis; in C. elegans, GPD-2 and GPD-3 constitute the major GAPDH isoenzymes and are expressed at highest levels during postembryonic development, primarily in the actin-containing A and I zones of body-wall muscle; loss of gpd-2 function via RNA-mediated interference (RNAi) does not result in any abnormalities. |
KOG Annotation  |
Glyceraldehyde 3-phosphate dehydrogenase
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Evidence for Inhibition |
Sliding Window |
Raw score: 506
Relative score: 0.567 (Best raw score= for Gene )
Specificity index: 0.263 |
Wormbase (BLAST) |
RNAi primary |
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External "K10B3.8" Links |
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[cgc5655]:daf-2:K10B3.7 Phenotypes |
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Phenotype |
Unclassified
lipid metabolism abnormal
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[cgc5655]:daf-2:K10B3.7 Experiment Details |
Ace View |
Laboratory |
GR |
RNAi Reagent & Type |
sjj_K10B3.7 (PCR_reagent) |
Strain |
daf-2(e1370) |
Reference |
Ashrafi K et al. (2003) Nature "Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory ...." |
Delivery Method |
Bacterial_feeding |
Date |
2003-1-10 |
Gene Plot of K10B3.9 |
Browse using GBrowse
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Gene Plot of K10B3.7 |
Browse using GBrowse
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Gene Plot of K10B3.8 |
Browse using GBrowse
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